| Parameters | Input values |
|---|---|
| Folder name : | Example data |
| Sequence name : | CDH1 |
| Group : | High methylated DNA |
| Experiment type : | Direct-BSP |
| Replicate number : | 2 |
| Date of sequencing #1 : | 2022-05-02 |
| Date of sequencing #2 : | 2022-05-02 |
| Date of analysis : | 2022-06-04 11:17 |
| Prefix of output files : | CDH1_High methylated DNA_2 |
| Reference DNA sequence information | Input values |
|---|---|
| Name | CDH1 |
| Strand used for primer design | Plus strand |
| Length | 341 pb |
| Coordinates on reference genome | chr16:68770940-68771280 |
## CCACCGGCGGGGCTGGGATTCGAACCCAGTGGAATCAGAACCGTGCAGGTCCCATAACCCACCTAGACCCTAGCAACTCCAGGCTAGAGGGTCACCGCGTCTATGCGAGGCCGGGTGGGCGGGCCGTCAGCTCCGCCCTGGGGAGGGGTCCGCGCTGCTGATTGGCTGTGGCCGGCAGGTGAACCCTCAGCCAATCAGCGGTACGGGGGGCGGTGCCTCCGGGGCTCACCTGGCTGCAGCCACGCACCCCCTCTCAGTGGCGTCGGAACTGCAAAGCACCTGTGAGCTTGCGGAAGTCAGTTCAGACTCCAGCCCGCTCCAGCCCGGCCCGACCCGACCGC
## GCGGTCGGGTCGGGCCGGGCTGGAGCGGGCTGGAGTCTGAACTGACTTCCGCAAGCTCACAGGTGCTTTGCAGTTCCGACGCCACTGAGAGGGGGTGCGTGGCTGCAGCCAGGTGAGCCCCGGAGGCACCGCCCCCCGTACCGCTGATTGGCTGAGGGTTCACCTGCCGGCCACAGCCAATCAGCAGCGCGGACCCCTCCCCAGGGCGGAGCTGACGGCCCGCCCACCCGGCCTCGCATAGACGCGGTGACCCTCTAGCCTGGAGTTGCTAGGGTCTAGGTGGGTTATGGGACCTGCACGGTTCTGATTCCACTGGGTTCGAATCCCAGCCCCGCCGGTGG
| CG number | chr | start | end | C position | G position |
|---|---|---|---|---|---|
| 1 | chr16 | 68770944 | 68770945 | 5 | 6 |
| 2 | chr16 | 68770947 | 68770948 | 8 | 9 |
| 3 | chr16 | 68770960 | 68770961 | 21 | 22 |
| 4 | chr16 | 68770981 | 68770982 | 42 | 43 |
| 5 | chr16 | 68771035 | 68771036 | 96 | 97 |
| 6 | chr16 | 68771037 | 68771038 | 98 | 99 |
| 7 | chr16 | 68771045 | 68771046 | 106 | 107 |
| 8 | chr16 | 68771051 | 68771052 | 112 | 113 |
| 9 | chr16 | 68771059 | 68771060 | 120 | 121 |
| 10 | chr16 | 68771064 | 68771065 | 125 | 126 |
| 11 | chr16 | 68771073 | 68771074 | 134 | 135 |
| 12 | chr16 | 68771090 | 68771091 | 151 | 152 |
| 13 | chr16 | 68771092 | 68771093 | 153 | 154 |
| 14 | chr16 | 68771112 | 68771113 | 173 | 174 |
| 15 | chr16 | 68771138 | 68771139 | 199 | 200 |
| 16 | chr16 | 68771143 | 68771144 | 204 | 205 |
| 17 | chr16 | 68771150 | 68771151 | 211 | 212 |
| 18 | chr16 | 68771159 | 68771160 | 220 | 221 |
| 19 | chr16 | 68771182 | 68771183 | 243 | 244 |
| 20 | chr16 | 68771200 | 68771201 | 261 | 262 |
| 21 | chr16 | 68771203 | 68771204 | 264 | 265 |
| 22 | chr16 | 68771230 | 68771231 | 291 | 292 |
| 23 | chr16 | 68771254 | 68771255 | 315 | 316 |
| 24 | chr16 | 68771264 | 68771265 | 325 | 326 |
| 25 | chr16 | 68771269 | 68771270 | 330 | 331 |
| 26 | chr16 | 68771274 | 68771275 | 335 | 336 |
| 27 | chr16 | 68771278 | 68771279 | 339 | 340 |
| CG number | chr | start | end | C position | G position |
|---|---|---|---|---|---|
| 27 | chr16 | 68771278 | 68771279 | 2 | 3 |
| 26 | chr16 | 68771274 | 68771275 | 6 | 7 |
| 25 | chr16 | 68771269 | 68771270 | 11 | 12 |
| 24 | chr16 | 68771264 | 68771265 | 16 | 17 |
| 23 | chr16 | 68771254 | 68771255 | 26 | 27 |
| 22 | chr16 | 68771230 | 68771231 | 50 | 51 |
| 21 | chr16 | 68771203 | 68771204 | 77 | 78 |
| 20 | chr16 | 68771200 | 68771201 | 80 | 81 |
| 19 | chr16 | 68771182 | 68771183 | 98 | 99 |
| 18 | chr16 | 68771159 | 68771160 | 121 | 122 |
| 17 | chr16 | 68771150 | 68771151 | 130 | 131 |
| 16 | chr16 | 68771143 | 68771144 | 137 | 138 |
| 15 | chr16 | 68771138 | 68771139 | 142 | 143 |
| 14 | chr16 | 68771112 | 68771113 | 168 | 169 |
| 13 | chr16 | 68771092 | 68771093 | 188 | 189 |
| 12 | chr16 | 68771090 | 68771091 | 190 | 191 |
| 11 | chr16 | 68771073 | 68771074 | 207 | 208 |
| 10 | chr16 | 68771064 | 68771065 | 216 | 217 |
| 9 | chr16 | 68771059 | 68771060 | 221 | 222 |
| 8 | chr16 | 68771051 | 68771052 | 229 | 230 |
| 7 | chr16 | 68771045 | 68771046 | 235 | 236 |
| 6 | chr16 | 68771037 | 68771038 | 243 | 244 |
| 5 | chr16 | 68771035 | 68771036 | 245 | 246 |
| 4 | chr16 | 68770981 | 68770982 | 299 | 300 |
| 3 | chr16 | 68770960 | 68770961 | 320 | 321 |
| 2 | chr16 | 68770947 | 68770948 | 333 | 334 |
| 1 | chr16 | 68770944 | 68770945 | 336 | 337 |
Bisulfite converted sequence of the plus strand as indicated as the template strand.
## TTATCGGCGGGGTTGGGATTCGAATTTAGTGGAATTAGAATCGTGTAGGTTTTATAATTTATTTAGATTTTAGTAATTTTAGGTTAGAGGGTTATCGCGTTTATGCGAGGTCGGGTGGGCGGGTCGTTAGTTTCGTTTTGGGGAGGGGTTCGCGTTGTTGATTGGTTGTGGTCGGTAGGTGAATTTTTAGTTAATTAGCGGTACGGGGGGCGGTGTTTTCGGGGTTTATTTGGTTGTAGTTACGTATTTTTTTTTAGTGGCGTCGGAATTGTAAAGTATTTGTGAGTTTGCGGAAGTTAGTTTAGATTTTAGTTCGTTTTAGTTCGGTTCGATTCGATCGT
Reverse complement of the bisulfite converted sequence from the plus strand.
## ACGATCGAATCGAACCGAACTAAAACGAACTAAAATCTAAACTAACTTCCGCAAACTCACAAATACTTTACAATTCCGACGCCACTAAAAAAAAATACGTAACTACAACCAAATAAACCCCGAAAACACCGCCCCCCGTACCGCTAATTAACTAAAAATTCACCTACCGACCACAACCAATCAACAACGCGAACCCCTCCCCAAAACGAAACTAACGACCCGCCCACCCGACCTCGCATAAACGCGATAACCCTCTAACCTAAAATTACTAAAATCTAAATAAATTATAAAACCTACACGATTCTAATTCCACTAAATTCGAATCCCAACCCCGCCGATAA
| Thresholds | Values |
|---|---|
| Minimum length of the trimmed sequence : | 30 bp |
| Minimum Phred quality score : | 30 |
| Minimum ratio of primary peak : | 0.75 |
| Minimum percentage of non-mixed positions : | 75 % |
| Sequencing | Length | Start position | End position | Mean of Phred quality scores | Mean of primary peak ratio | Percentage of non-mixed positions | Trimmed sequence quality |
|---|---|---|---|---|---|---|---|
| #1 | 128 | 24 | 151 | 35.45 | 0.82 | 73.44 | Incorrect |
| #2 | 198 | 25 | 222 | 48.49 | 0.95 | 93.43 | Correct |
Sequence of primary peaks:
Length: 348 bp
## TNAATTAGGGTACGTCAGTAGACGATATTGCGATATGCGAAGTCGGGTGGGCGGGTCGTTAGTTTCGTTATGGGGAGGGGTTCGCGTTGTTGATTGGTTGTGGTCGGTAGGTGAATTTTTAGTTAATTAGCGGTACGGGGGGCGGTGTTTTCGGGGTTTATTTGGTTGTAGTTACGTATTTTTTTTTAGTGGNGTCNGAATTGTAAANTATTTGTGAGTTTCCTCGACTGTGCCTACTAAGTTAATNTGANCCTAACTATCGCGCGGGNAGTTATATGANNANTCTATNTTANGATACTATCNCTCNCTTGNTTANAGNTTNNTATTTCCGTACCANCTANNATGCCC
Open chromatogram file in a new tab
| Position | A peak | T peak | G peak | C peak | Total | A ratio | T ratio | G ratio | C ratio | Primary base | Quality score | Primary ratio |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 52 | 142 | 19 | 19 | 232 | 0.22 | 0.61 | 0.08 | 0.08 | T | 1 | 0.61 |
| 2 | 35 | 54 | 20 | 22 | 131 | 0.27 | 0.41 | 0.15 | 0.17 | N | 3 | 0.41 |
| 3 | 92 | 37 | 7 | 15 | 151 | 0.61 | 0.25 | 0.05 | 0.10 | A | 3 | 0.61 |
| 4 | 74 | 0 | 1 | 4 | 79 | 0.94 | 0.00 | 0.01 | 0.05 | A | 3 | 0.94 |
| 5 | 23 | 41 | 4 | 26 | 94 | 0.24 | 0.44 | 0.04 | 0.28 | T | 4 | 0.44 |
| 6 | 1 | 74 | 0 | 7 | 82 | 0.01 | 0.90 | 0.00 | 0.09 | T | 9 | 0.90 |
| 7 | 72 | 55 | 1 | 29 | 157 | 0.46 | 0.35 | 0.01 | 0.18 | A | 5 | 0.46 |
| 8 | 29 | 0 | 273 | 24 | 326 | 0.09 | 0.00 | 0.84 | 0.07 | G | 9 | 0.84 |
| 9 | 7 | 0 | 151 | 23 | 181 | 0.04 | 0.00 | 0.83 | 0.13 | G | 4 | 0.83 |
| 10 | 17 | 1 | 175 | 10 | 203 | 0.08 | 0.00 | 0.86 | 0.05 | G | 4 | 0.86 |
| 11 | 1 | 51 | 0 | 22 | 74 | 0.01 | 0.69 | 0.00 | 0.30 | T | 5 | 0.69 |
| 12 | 47 | 0 | 1 | 22 | 70 | 0.67 | 0.00 | 0.01 | 0.31 | A | 5 | 0.67 |
| 13 | 0 | 0 | 0 | 60 | 60 | 0.00 | 0.00 | 0.00 | 1.00 | C | 4 | 1.00 |
| 14 | 0 | 0 | 1401 | 60 | 1461 | 0.00 | 0.00 | 0.96 | 0.04 | G | 5 | 0.96 |
| 15 | 6860 | 13175 | 0 | 0 | 20035 | 0.34 | 0.66 | 0.00 | 0.00 | T | 4 | 0.66 |
| 16 | 0 | 0 | 0 | 17 | 17 | 0.00 | 0.00 | 0.00 | 1.00 | C | 5 | 1.00 |
| 17 | 3789 | 0 | 0 | 0 | 3789 | 1.00 | 0.00 | 0.00 | 0.00 | A | 5 | 1.00 |
| 18 | 0 | 0 | 4182 | 2 | 4184 | 0.00 | 0.00 | 1.00 | 0.00 | G | 27 | 1.00 |
| 19 | 0 | 3831 | 0 | 40 | 3871 | 0.00 | 0.99 | 0.00 | 0.01 | T | 5 | 0.99 |
| 20 | 2805 | 0 | 0 | 38 | 2843 | 0.99 | 0.00 | 0.00 | 0.01 | A | 4 | 0.99 |
| 21 | 0 | 0 | 1062 | 0 | 1062 | 0.00 | 0.00 | 1.00 | 0.00 | G | 6 | 1.00 |
| 22 | 3153 | 42 | 0 | 0 | 3195 | 0.99 | 0.01 | 0.00 | 0.00 | A | 16 | 0.99 |
| 23 | 0 | 42 | 0 | 71 | 113 | 0.00 | 0.37 | 0.00 | 0.63 | C | 9 | 0.63 |
| 24 | 0 | 55 | 2806 | 22 | 2883 | 0.00 | 0.02 | 0.97 | 0.01 | G | 38 | 0.97 |
| 25 | 213 | 0 | 0 | 25 | 238 | 0.89 | 0.00 | 0.00 | 0.11 | A | 4 | 0.89 |
| 26 | 0 | 1319 | 37 | 32 | 1388 | 0.00 | 0.95 | 0.03 | 0.02 | T | 5 | 0.95 |
| 27 | 1417 | 0 | 0 | 36 | 1453 | 0.98 | 0.00 | 0.00 | 0.02 | A | 13 | 0.98 |
| 28 | 0 | 1154 | 39 | 0 | 1193 | 0.00 | 0.97 | 0.03 | 0.00 | T | 10 | 0.97 |
| 29 | 0 | 824 | 0 | 557 | 1381 | 0.00 | 0.60 | 0.00 | 0.40 | T | 9 | 0.60 |
| 30 | 111 | 0 | 1064 | 0 | 1175 | 0.09 | 0.00 | 0.91 | 0.00 | G | 13 | 0.91 |
| 31 | 0 | 57 | 0 | 370 | 427 | 0.00 | 0.13 | 0.00 | 0.87 | C | 8 | 0.87 |
| 32 | 0 | 0 | 495 | 42 | 537 | 0.00 | 0.00 | 0.92 | 0.08 | G | 8 | 0.92 |
| 33 | 655 | 0 | 8 | 8 | 671 | 0.98 | 0.00 | 0.01 | 0.01 | A | 4 | 0.98 |
| 34 | 0 | 774 | 11 | 28 | 813 | 0.00 | 0.95 | 0.01 | 0.03 | T | 5 | 0.95 |
| 35 | 494 | 0 | 425 | 0 | 919 | 0.54 | 0.00 | 0.46 | 0.00 | A | 4 | 0.54 |
| 36 | 0 | 503 | 0 | 49 | 552 | 0.00 | 0.91 | 0.00 | 0.09 | T | 7 | 0.91 |
| 37 | 262 | 0 | 454 | 0 | 716 | 0.37 | 0.00 | 0.63 | 0.00 | G | 9 | 0.63 |
| 38 | 266 | 32 | 0 | 423 | 721 | 0.37 | 0.04 | 0.00 | 0.59 | C | 5 | 0.59 |
| 39 | 0 | 1 | 374 | 5 | 380 | 0.00 | 0.00 | 0.98 | 0.01 | G | 11 | 0.98 |
| 40 | 454 | 0 | 0 | 14 | 468 | 0.97 | 0.00 | 0.00 | 0.03 | A | 16 | 0.97 |
| 41 | 240 | 0 | 234 | 0 | 474 | 0.51 | 0.00 | 0.49 | 0.00 | A | 10 | 0.51 |
| 42 | 0 | 189 | 496 | 10 | 695 | 0.00 | 0.27 | 0.71 | 0.01 | G | 19 | 0.71 |
| 43 | 48 | 453 | 2 | 0 | 503 | 0.10 | 0.90 | 0.00 | 0.00 | T | 8 | 0.90 |
| 44 | 34 | 201 | 1 | 476 | 712 | 0.05 | 0.28 | 0.00 | 0.67 | C | 13 | 0.67 |
| 45 | 38 | 0 | 378 | 7 | 423 | 0.09 | 0.00 | 0.89 | 0.02 | G | 12 | 0.89 |
| 46 | 11 | 115 | 358 | 2 | 486 | 0.02 | 0.24 | 0.74 | 0.00 | G | 12 | 0.74 |
| 47 | 3 | 0 | 336 | 54 | 393 | 0.01 | 0.00 | 0.85 | 0.14 | G | 14 | 0.85 |
| 48 | 0 | 274 | 0 | 9 | 283 | 0.00 | 0.97 | 0.00 | 0.03 | T | 14 | 0.97 |
| 49 | 182 | 3 | 804 | 7 | 996 | 0.18 | 0.00 | 0.81 | 0.01 | G | 41 | 0.81 |
| 50 | 0 | 107 | 315 | 27 | 449 | 0.00 | 0.24 | 0.70 | 0.06 | G | 22 | 0.70 |
| 51 | 44 | 107 | 251 | 0 | 402 | 0.11 | 0.27 | 0.62 | 0.00 | G | 16 | 0.62 |
| 52 | 12 | 92 | 0 | 337 | 441 | 0.03 | 0.21 | 0.00 | 0.76 | C | 21 | 0.76 |
| 53 | 2 | 0 | 393 | 133 | 528 | 0.00 | 0.00 | 0.74 | 0.25 | G | 24 | 0.74 |
| 54 | 15 | 34 | 334 | 96 | 479 | 0.03 | 0.07 | 0.70 | 0.20 | G | 18 | 0.70 |
| 55 | 26 | 132 | 177 | 13 | 348 | 0.07 | 0.38 | 0.51 | 0.04 | G | 10 | 0.51 |
| 56 | 2 | 315 | 2 | 132 | 451 | 0.00 | 0.70 | 0.00 | 0.29 | T | 14 | 0.70 |
| 57 | 23 | 0 | 0 | 490 | 513 | 0.04 | 0.00 | 0.00 | 0.96 | C | 31 | 0.96 |
| 58 | 103 | 36 | 284 | 2 | 425 | 0.24 | 0.08 | 0.67 | 0.00 | G | 14 | 0.67 |
| 59 | 32 | 295 | 1 | 91 | 419 | 0.08 | 0.70 | 0.00 | 0.22 | T | 16 | 0.70 |
| 60 | 0 | 512 | 13 | 30 | 555 | 0.00 | 0.92 | 0.02 | 0.05 | T | 22 | 0.92 |
| 61 | 366 | 27 | 228 | 29 | 650 | 0.56 | 0.04 | 0.35 | 0.04 | A | 22 | 0.56 |
| 62 | 13 | 61 | 234 | 52 | 360 | 0.04 | 0.17 | 0.65 | 0.14 | G | 10 | 0.65 |
| 63 | 14 | 306 | 136 | 0 | 456 | 0.03 | 0.67 | 0.30 | 0.00 | T | 15 | 0.67 |
| 64 | 0 | 431 | 0 | 114 | 545 | 0.00 | 0.79 | 0.00 | 0.21 | T | 22 | 0.79 |
| 65 | 0 | 347 | 8 | 0 | 355 | 0.00 | 0.98 | 0.02 | 0.00 | T | 23 | 0.98 |
| 66 | 36 | 82 | 2 | 478 | 598 | 0.06 | 0.14 | 0.00 | 0.80 | C | 38 | 0.80 |
| 67 | 25 | 0 | 245 | 12 | 282 | 0.09 | 0.00 | 0.87 | 0.04 | G | 12 | 0.87 |
| 68 | 28 | 291 | 0 | 141 | 460 | 0.06 | 0.63 | 0.00 | 0.31 | T | 10 | 0.63 |
| 69 | 55 | 497 | 0 | 21 | 573 | 0.10 | 0.87 | 0.00 | 0.04 | T | 13 | 0.87 |
| 70 | 506 | 387 | 8 | 5 | 906 | 0.56 | 0.43 | 0.01 | 0.01 | A | 10 | 0.56 |
| 71 | 170 | 378 | 4 | 2 | 554 | 0.31 | 0.68 | 0.01 | 0.00 | T | 11 | 0.68 |
| 72 | 0 | 3 | 547 | 28 | 578 | 0.00 | 0.01 | 0.95 | 0.05 | G | 15 | 0.95 |
| 73 | 255 | 13 | 289 | 0 | 557 | 0.46 | 0.02 | 0.52 | 0.00 | G | 13 | 0.52 |
| 74 | 21 | 7 | 296 | 67 | 391 | 0.05 | 0.02 | 0.76 | 0.17 | G | 13 | 0.76 |
| 75 | 31 | 7 | 314 | 43 | 395 | 0.08 | 0.02 | 0.79 | 0.11 | G | 19 | 0.79 |
| 76 | 577 | 29 | 0 | 4 | 610 | 0.95 | 0.05 | 0.00 | 0.01 | A | 59 | 0.95 |
| 77 | 12 | 2 | 326 | 6 | 346 | 0.03 | 0.01 | 0.94 | 0.02 | G | 39 | 0.94 |
| 78 | 64 | 1 | 458 | 0 | 523 | 0.12 | 0.00 | 0.88 | 0.00 | G | 56 | 0.88 |
| 79 | 15 | 26 | 368 | 0 | 409 | 0.04 | 0.06 | 0.90 | 0.00 | G | 29 | 0.90 |
| 80 | 0 | 58 | 384 | 0 | 442 | 0.00 | 0.13 | 0.87 | 0.00 | G | 39 | 0.87 |
| 81 | 7 | 407 | 3 | 0 | 417 | 0.02 | 0.98 | 0.01 | 0.00 | T | 24 | 0.98 |
| 82 | 9 | 436 | 1 | 0 | 446 | 0.02 | 0.98 | 0.00 | 0.00 | T | 30 | 0.98 |
| 83 | 15 | 23 | 1 | 700 | 739 | 0.02 | 0.03 | 0.00 | 0.95 | C | 39 | 0.95 |
| 84 | 18 | 32 | 299 | 224 | 573 | 0.03 | 0.06 | 0.52 | 0.39 | G | 18 | 0.52 |
| 85 | 32 | 0 | 40 | 456 | 528 | 0.06 | 0.00 | 0.08 | 0.86 | C | 21 | 0.86 |
| 86 | 53 | 115 | 467 | 93 | 728 | 0.07 | 0.16 | 0.64 | 0.13 | G | 28 | 0.64 |
| 87 | 87 | 424 | 20 | 35 | 566 | 0.15 | 0.75 | 0.04 | 0.06 | T | 36 | 0.75 |
| 88 | 0 | 432 | 0 | 35 | 467 | 0.00 | 0.93 | 0.00 | 0.07 | T | 36 | 0.93 |
| 89 | 98 | 46 | 461 | 37 | 642 | 0.15 | 0.07 | 0.72 | 0.06 | G | 40 | 0.72 |
| 90 | 0 | 270 | 61 | 12 | 343 | 0.00 | 0.79 | 0.18 | 0.03 | T | 34 | 0.79 |
| 91 | 59 | 411 | 41 | 32 | 543 | 0.11 | 0.76 | 0.08 | 0.06 | T | 48 | 0.76 |
| 92 | 39 | 45 | 438 | 3 | 525 | 0.07 | 0.09 | 0.83 | 0.01 | G | 59 | 0.83 |
| 93 | 494 | 25 | 0 | 51 | 570 | 0.87 | 0.04 | 0.00 | 0.09 | A | 59 | 0.87 |
| 94 | 26 | 338 | 38 | 0 | 402 | 0.06 | 0.84 | 0.09 | 0.00 | T | 59 | 0.84 |
| 95 | 30 | 436 | 23 | 23 | 512 | 0.06 | 0.85 | 0.04 | 0.04 | T | 56 | 0.85 |
| 96 | 8 | 27 | 697 | 0 | 732 | 0.01 | 0.04 | 0.95 | 0.00 | G | 62 | 0.95 |
| 97 | 19 | 13 | 246 | 15 | 293 | 0.06 | 0.04 | 0.84 | 0.05 | G | 59 | 0.84 |
| 98 | 28 | 245 | 17 | 14 | 304 | 0.09 | 0.81 | 0.06 | 0.05 | T | 50 | 0.81 |
| 99 | 0 | 415 | 4 | 25 | 444 | 0.00 | 0.93 | 0.01 | 0.06 | T | 56 | 0.93 |
| 100 | 34 | 8 | 527 | 1 | 570 | 0.06 | 0.01 | 0.92 | 0.00 | G | 54 | 0.92 |
| 101 | 58 | 264 | 10 | 23 | 355 | 0.16 | 0.74 | 0.03 | 0.06 | T | 50 | 0.74 |
| 102 | 0 | 7 | 534 | 19 | 560 | 0.00 | 0.01 | 0.95 | 0.03 | G | 62 | 0.95 |
| 103 | 19 | 28 | 313 | 8 | 368 | 0.05 | 0.08 | 0.85 | 0.02 | G | 43 | 0.85 |
| 104 | 38 | 275 | 4 | 5 | 322 | 0.12 | 0.85 | 0.01 | 0.02 | T | 59 | 0.85 |
| 105 | 11 | 4 | 0 | 454 | 469 | 0.02 | 0.01 | 0.00 | 0.97 | C | 59 | 0.97 |
| 106 | 5 | 38 | 279 | 17 | 339 | 0.01 | 0.11 | 0.82 | 0.05 | G | 54 | 0.82 |
| 107 | 0 | 0 | 315 | 14 | 329 | 0.00 | 0.00 | 0.96 | 0.04 | G | 42 | 0.96 |
| 108 | 27 | 217 | 2 | 0 | 246 | 0.11 | 0.88 | 0.01 | 0.00 | T | 59 | 0.88 |
| 109 | 381 | 3 | 0 | 28 | 412 | 0.92 | 0.01 | 0.00 | 0.07 | A | 62 | 0.92 |
| 110 | 21 | 1 | 206 | 18 | 246 | 0.09 | 0.00 | 0.84 | 0.07 | G | 43 | 0.84 |
| 111 | 10 | 1 | 389 | 0 | 400 | 0.03 | 0.00 | 0.97 | 0.00 | G | 59 | 0.97 |
| 112 | 17 | 256 | 8 | 65 | 346 | 0.05 | 0.74 | 0.02 | 0.19 | T | 46 | 0.74 |
| 113 | 2 | 9 | 460 | 0 | 471 | 0.00 | 0.02 | 0.98 | 0.00 | G | 56 | 0.98 |
| 114 | 439 | 29 | 18 | 28 | 514 | 0.85 | 0.06 | 0.04 | 0.05 | A | 54 | 0.85 |
| 115 | 348 | 0 | 33 | 0 | 381 | 0.91 | 0.00 | 0.09 | 0.00 | A | 56 | 0.91 |
| 116 | 0 | 333 | 9 | 48 | 390 | 0.00 | 0.85 | 0.02 | 0.12 | T | 59 | 0.85 |
| 117 | 31 | 527 | 19 | 20 | 597 | 0.05 | 0.88 | 0.03 | 0.03 | T | 62 | 0.88 |
| 118 | 35 | 372 | 11 | 0 | 418 | 0.08 | 0.89 | 0.03 | 0.00 | T | 59 | 0.89 |
| 119 | 44 | 342 | 0 | 56 | 442 | 0.10 | 0.77 | 0.00 | 0.13 | T | 59 | 0.77 |
| 120 | 0 | 332 | 9 | 6 | 347 | 0.00 | 0.96 | 0.03 | 0.02 | T | 59 | 0.96 |
| 121 | 414 | 5 | 12 | 26 | 457 | 0.91 | 0.01 | 0.03 | 0.06 | A | 54 | 0.91 |
| 122 | 53 | 9 | 171 | 27 | 260 | 0.20 | 0.03 | 0.66 | 0.10 | G | 34 | 0.66 |
| 123 | 23 | 257 | 10 | 0 | 290 | 0.08 | 0.89 | 0.03 | 0.00 | T | 49 | 0.89 |
| 124 | 14 | 352 | 1 | 24 | 391 | 0.04 | 0.90 | 0.00 | 0.06 | T | 49 | 0.90 |
| 125 | 302 | 7 | 24 | 68 | 401 | 0.75 | 0.02 | 0.06 | 0.17 | A | 50 | 0.75 |
| 126 | 294 | 4 | 7 | 0 | 305 | 0.96 | 0.01 | 0.02 | 0.00 | A | 50 | 0.96 |
| 127 | 17 | 270 | 0 | 64 | 351 | 0.05 | 0.77 | 0.00 | 0.18 | T | 46 | 0.77 |
| 128 | 47 | 387 | 20 | 37 | 491 | 0.10 | 0.79 | 0.04 | 0.08 | T | 46 | 0.79 |
| 129 | 387 | 24 | 12 | 15 | 438 | 0.88 | 0.05 | 0.03 | 0.03 | A | 54 | 0.88 |
| 130 | 33 | 28 | 118 | 47 | 226 | 0.15 | 0.12 | 0.52 | 0.21 | G | 23 | 0.52 |
| 131 | 33 | 28 | 21 | 229 | 311 | 0.11 | 0.09 | 0.07 | 0.74 | C | 48 | 0.74 |
| 132 | 19 | 45 | 312 | 28 | 404 | 0.05 | 0.11 | 0.77 | 0.07 | G | 56 | 0.77 |
| 133 | 0 | 46 | 245 | 0 | 291 | 0.00 | 0.16 | 0.84 | 0.00 | G | 56 | 0.84 |
| 134 | 29 | 232 | 4 | 45 | 310 | 0.09 | 0.75 | 0.01 | 0.15 | T | 49 | 0.75 |
| 135 | 303 | 46 | 14 | 8 | 371 | 0.82 | 0.12 | 0.04 | 0.02 | A | 59 | 0.82 |
| 136 | 37 | 38 | 7 | 264 | 346 | 0.11 | 0.11 | 0.02 | 0.76 | C | 59 | 0.76 |
| 137 | 34 | 1 | 238 | 18 | 291 | 0.12 | 0.00 | 0.82 | 0.06 | G | 50 | 0.82 |
| 138 | 14 | 0 | 364 | 38 | 416 | 0.03 | 0.00 | 0.88 | 0.09 | G | 59 | 0.88 |
| 139 | 2 | 43 | 231 | 49 | 325 | 0.01 | 0.13 | 0.71 | 0.15 | G | 51 | 0.71 |
| 140 | 10 | 34 | 291 | 0 | 335 | 0.03 | 0.10 | 0.87 | 0.00 | G | 59 | 0.87 |
| 141 | 24 | 24 | 296 | 10 | 354 | 0.07 | 0.07 | 0.84 | 0.03 | G | 59 | 0.84 |
| 142 | 38 | 17 | 278 | 45 | 378 | 0.10 | 0.04 | 0.74 | 0.12 | G | 56 | 0.74 |
| 143 | 45 | 0 | 11 | 226 | 282 | 0.16 | 0.00 | 0.04 | 0.80 | C | 51 | 0.80 |
| 144 | 21 | 33 | 322 | 6 | 382 | 0.05 | 0.09 | 0.84 | 0.02 | G | 59 | 0.84 |
| 145 | 0 | 4 | 269 | 54 | 327 | 0.00 | 0.01 | 0.82 | 0.17 | G | 56 | 0.82 |
| 146 | 41 | 184 | 8 | 1 | 234 | 0.18 | 0.79 | 0.03 | 0.00 | T | 49 | 0.79 |
| 147 | 0 | 16 | 486 | 1 | 503 | 0.00 | 0.03 | 0.97 | 0.00 | G | 59 | 0.97 |
| 148 | 7 | 266 | 12 | 4 | 289 | 0.02 | 0.92 | 0.04 | 0.01 | T | 59 | 0.92 |
| 149 | 15 | 350 | 5 | 25 | 395 | 0.04 | 0.89 | 0.01 | 0.06 | T | 50 | 0.89 |
| 150 | 49 | 271 | 13 | 36 | 369 | 0.13 | 0.73 | 0.04 | 0.10 | T | 46 | 0.73 |
| 151 | 36 | 304 | 10 | 6 | 356 | 0.10 | 0.85 | 0.03 | 0.02 | T | 34 | 0.85 |
| 152 | 76 | 71 | 30 | 271 | 448 | 0.17 | 0.16 | 0.07 | 0.60 | C | 21 | 0.60 |
| 153 | 87 | 109 | 246 | 39 | 481 | 0.18 | 0.23 | 0.51 | 0.08 | G | 13 | 0.51 |
| 154 | 163 | 99 | 464 | 45 | 771 | 0.21 | 0.13 | 0.60 | 0.06 | G | 26 | 0.60 |
| 155 | 211 | 110 | 431 | 55 | 807 | 0.26 | 0.14 | 0.53 | 0.07 | G | 25 | 0.53 |
| 156 | 0 | 0 | 451 | 0 | 451 | 0.00 | 0.00 | 1.00 | 0.00 | G | 26 | 1.00 |
| 157 | 92 | 232 | 76 | 53 | 453 | 0.20 | 0.51 | 0.17 | 0.12 | T | 24 | 0.51 |
| 158 | 0 | 279 | 0 | 18 | 297 | 0.00 | 0.94 | 0.00 | 0.06 | T | 28 | 0.94 |
| 159 | 25 | 251 | 28 | 0 | 304 | 0.08 | 0.83 | 0.09 | 0.00 | T | 34 | 0.83 |
| 160 | 355 | 6 | 0 | 53 | 414 | 0.86 | 0.01 | 0.00 | 0.13 | A | 56 | 0.86 |
| 161 | 7 | 230 | 23 | 60 | 320 | 0.02 | 0.72 | 0.07 | 0.19 | T | 51 | 0.72 |
| 162 | 36 | 271 | 26 | 10 | 343 | 0.10 | 0.79 | 0.08 | 0.03 | T | 50 | 0.79 |
| 163 | 0 | 273 | 31 | 4 | 308 | 0.00 | 0.89 | 0.10 | 0.01 | T | 48 | 0.89 |
| 164 | 92 | 6 | 330 | 0 | 428 | 0.21 | 0.01 | 0.77 | 0.00 | G | 32 | 0.77 |
| 165 | 5 | 5 | 221 | 11 | 242 | 0.02 | 0.02 | 0.91 | 0.05 | G | 28 | 0.91 |
| 166 | 41 | 180 | 0 | 20 | 241 | 0.17 | 0.75 | 0.00 | 0.08 | T | 36 | 0.75 |
| 167 | 16 | 277 | 60 | 47 | 400 | 0.04 | 0.69 | 0.15 | 0.12 | T | 59 | 0.69 |
| 168 | 2 | 7 | 342 | 23 | 374 | 0.01 | 0.02 | 0.91 | 0.06 | G | 50 | 0.91 |
| 169 | 23 | 155 | 19 | 67 | 264 | 0.09 | 0.59 | 0.07 | 0.25 | T | 39 | 0.59 |
| 170 | 216 | 24 | 19 | 7 | 266 | 0.81 | 0.09 | 0.07 | 0.03 | A | 43 | 0.81 |
| 171 | 42 | 26 | 98 | 1 | 167 | 0.25 | 0.16 | 0.59 | 0.01 | G | 18 | 0.59 |
| 172 | 22 | 174 | 19 | 30 | 245 | 0.09 | 0.71 | 0.08 | 0.12 | T | 28 | 0.71 |
| 173 | 14 | 261 | 9 | 2 | 286 | 0.05 | 0.91 | 0.03 | 0.01 | T | 46 | 0.91 |
| 174 | 197 | 21 | 30 | 0 | 248 | 0.79 | 0.08 | 0.12 | 0.00 | A | 34 | 0.79 |
| 175 | 47 | 53 | 2 | 215 | 317 | 0.15 | 0.17 | 0.01 | 0.68 | C | 31 | 0.68 |
| 176 | 27 | 31 | 124 | 27 | 209 | 0.13 | 0.15 | 0.59 | 0.13 | G | 18 | 0.59 |
| 177 | 52 | 188 | 18 | 1 | 259 | 0.20 | 0.73 | 0.07 | 0.00 | T | 31 | 0.73 |
| 178 | 210 | 4 | 34 | 1 | 249 | 0.84 | 0.02 | 0.14 | 0.00 | A | 35 | 0.84 |
| 179 | 65 | 213 | 0 | 12 | 290 | 0.22 | 0.73 | 0.00 | 0.04 | T | 49 | 0.73 |
| 180 | 0 | 281 | 5 | 5 | 291 | 0.00 | 0.97 | 0.02 | 0.02 | T | 48 | 0.97 |
| 181 | 42 | 254 | 11 | 8 | 315 | 0.13 | 0.81 | 0.03 | 0.03 | T | 43 | 0.81 |
| 182 | 12 | 241 | 20 | 35 | 308 | 0.04 | 0.78 | 0.06 | 0.11 | T | 59 | 0.78 |
| 183 | 52 | 236 | 0 | 22 | 310 | 0.17 | 0.76 | 0.00 | 0.07 | T | 59 | 0.76 |
| 184 | 13 | 250 | 13 | 2 | 278 | 0.05 | 0.90 | 0.05 | 0.01 | T | 59 | 0.90 |
| 185 | 21 | 242 | 14 | 44 | 321 | 0.07 | 0.75 | 0.04 | 0.14 | T | 59 | 0.75 |
| 186 | 11 | 216 | 46 | 18 | 291 | 0.04 | 0.74 | 0.16 | 0.06 | T | 51 | 0.74 |
| 187 | 0 | 180 | 7 | 1 | 188 | 0.00 | 0.96 | 0.04 | 0.01 | T | 49 | 0.96 |
| 188 | 178 | 6 | 19 | 13 | 216 | 0.82 | 0.03 | 0.09 | 0.06 | A | 38 | 0.82 |
| 189 | 29 | 41 | 65 | 3 | 138 | 0.21 | 0.30 | 0.47 | 0.02 | G | 13 | 0.47 |
| 190 | 19 | 112 | 43 | 1 | 175 | 0.11 | 0.64 | 0.25 | 0.01 | T | 25 | 0.64 |
| 191 | 40 | 2 | 246 | 16 | 304 | 0.13 | 0.01 | 0.81 | 0.05 | G | 32 | 0.81 |
| 192 | 0 | 35 | 97 | 18 | 150 | 0.00 | 0.23 | 0.65 | 0.12 | G | 20 | 0.65 |
| 193 | 49 | 35 | 31 | 94 | 209 | 0.23 | 0.17 | 0.15 | 0.45 | N | 19 | 0.45 |
| 194 | 41 | 11 | 107 | 20 | 179 | 0.23 | 0.06 | 0.60 | 0.11 | G | 19 | 0.60 |
| 195 | 6 | 128 | 28 | 67 | 229 | 0.03 | 0.56 | 0.12 | 0.29 | T | 27 | 0.56 |
| 196 | 2 | 5 | 21 | 174 | 202 | 0.01 | 0.02 | 0.10 | 0.86 | C | 34 | 0.86 |
| 197 | 29 | 36 | 87 | 43 | 195 | 0.15 | 0.18 | 0.45 | 0.22 | N | 13 | 0.45 |
| 198 | 45 | 1 | 137 | 64 | 247 | 0.18 | 0.00 | 0.55 | 0.26 | G | 27 | 0.55 |
| 199 | 241 | 30 | 5 | 7 | 283 | 0.85 | 0.11 | 0.02 | 0.02 | A | 31 | 0.85 |
| 200 | 119 | 15 | 13 | 61 | 208 | 0.57 | 0.07 | 0.06 | 0.29 | A | 23 | 0.57 |
| 201 | 46 | 146 | 7 | 56 | 255 | 0.18 | 0.57 | 0.03 | 0.22 | T | 34 | 0.57 |
| 202 | 0 | 185 | 40 | 0 | 225 | 0.00 | 0.82 | 0.18 | 0.00 | T | 38 | 0.82 |
| 203 | 57 | 36 | 181 | 6 | 280 | 0.20 | 0.13 | 0.65 | 0.02 | G | 34 | 0.65 |
| 204 | 52 | 114 | 11 | 0 | 177 | 0.29 | 0.64 | 0.06 | 0.00 | T | 27 | 0.64 |
| 205 | 162 | 37 | 0 | 13 | 212 | 0.76 | 0.17 | 0.00 | 0.06 | A | 34 | 0.76 |
| 206 | 122 | 55 | 7 | 9 | 193 | 0.63 | 0.28 | 0.04 | 0.05 | A | 27 | 0.63 |
| 207 | 163 | 43 | 24 | 1 | 231 | 0.71 | 0.19 | 0.10 | 0.00 | A | 34 | 0.71 |
| 208 | 29 | 47 | 102 | 32 | 210 | 0.14 | 0.22 | 0.49 | 0.15 | N | 18 | 0.49 |
| 209 | 71 | 83 | 7 | 1 | 162 | 0.44 | 0.51 | 0.04 | 0.01 | T | 13 | 0.51 |
| 210 | 167 | 37 | 0 | 35 | 239 | 0.70 | 0.15 | 0.00 | 0.15 | A | 30 | 0.70 |
| 211 | 22 | 158 | 49 | 18 | 247 | 0.09 | 0.64 | 0.20 | 0.07 | T | 34 | 0.64 |
| 212 | 19 | 157 | 11 | 46 | 233 | 0.08 | 0.67 | 0.05 | 0.20 | T | 34 | 0.67 |
| 213 | 42 | 163 | 26 | 0 | 231 | 0.18 | 0.71 | 0.11 | 0.00 | T | 30 | 0.71 |
| 214 | 80 | 18 | 168 | 8 | 274 | 0.29 | 0.07 | 0.61 | 0.03 | G | 34 | 0.61 |
| 215 | 10 | 82 | 44 | 67 | 203 | 0.05 | 0.40 | 0.22 | 0.33 | T | 19 | 0.40 |
| 216 | 17 | 32 | 147 | 39 | 235 | 0.07 | 0.14 | 0.63 | 0.17 | G | 18 | 0.63 |
| 217 | 182 | 0 | 27 | 55 | 264 | 0.69 | 0.00 | 0.10 | 0.21 | A | 30 | 0.69 |
| 218 | 15 | 92 | 96 | 2 | 205 | 0.07 | 0.45 | 0.47 | 0.01 | G | 12 | 0.47 |
| 219 | 14 | 184 | 18 | 18 | 234 | 0.06 | 0.79 | 0.08 | 0.08 | T | 27 | 0.79 |
| 220 | 8 | 171 | 6 | 25 | 210 | 0.04 | 0.81 | 0.03 | 0.12 | T | 27 | 0.81 |
| 221 | 12 | 136 | 4 | 31 | 183 | 0.07 | 0.74 | 0.02 | 0.17 | T | 28 | 0.74 |
| 222 | 45 | 4 | 6 | 155 | 210 | 0.21 | 0.02 | 0.03 | 0.74 | C | 27 | 0.74 |
| 223 | 10 | 52 | 7 | 121 | 190 | 0.05 | 0.27 | 0.04 | 0.64 | C | 23 | 0.64 |
| 224 | 20 | 102 | 49 | 42 | 213 | 0.09 | 0.48 | 0.23 | 0.20 | T | 23 | 0.48 |
| 225 | 11 | 2 | 25 | 210 | 248 | 0.04 | 0.01 | 0.10 | 0.85 | C | 43 | 0.85 |
| 226 | 27 | 5 | 93 | 10 | 135 | 0.20 | 0.04 | 0.69 | 0.07 | G | 18 | 0.69 |
| 227 | 128 | 49 | 31 | 22 | 230 | 0.56 | 0.21 | 0.13 | 0.10 | A | 22 | 0.56 |
| 228 | 21 | 55 | 27 | 112 | 215 | 0.10 | 0.26 | 0.13 | 0.52 | C | 22 | 0.52 |
| 229 | 4 | 130 | 47 | 57 | 238 | 0.02 | 0.55 | 0.20 | 0.24 | T | 27 | 0.55 |
| 230 | 22 | 49 | 294 | 0 | 365 | 0.06 | 0.13 | 0.81 | 0.00 | G | 44 | 0.81 |
| 231 | 29 | 143 | 40 | 17 | 229 | 0.13 | 0.62 | 0.17 | 0.07 | T | 33 | 0.62 |
| 232 | 17 | 58 | 160 | 22 | 257 | 0.07 | 0.23 | 0.62 | 0.09 | G | 27 | 0.62 |
| 233 | 9 | 29 | 10 | 198 | 246 | 0.04 | 0.12 | 0.04 | 0.80 | C | 12 | 0.80 |
| 234 | 0 | 58 | 26 | 114 | 198 | 0.00 | 0.29 | 0.13 | 0.58 | C | 10 | 0.58 |
| 235 | 72 | 123 | 44 | 29 | 268 | 0.27 | 0.46 | 0.16 | 0.11 | T | 10 | 0.46 |
| 236 | 192 | 172 | 0 | 41 | 405 | 0.47 | 0.42 | 0.00 | 0.10 | A | 13 | 0.47 |
| 237 | 58 | 33 | 21 | 164 | 276 | 0.21 | 0.12 | 0.08 | 0.59 | C | 10 | 0.59 |
| 238 | 75 | 125 | 15 | 3 | 218 | 0.34 | 0.57 | 0.07 | 0.01 | T | 9 | 0.57 |
| 239 | 340 | 21 | 15 | 17 | 393 | 0.87 | 0.05 | 0.04 | 0.04 | A | 44 | 0.87 |
| 240 | 190 | 0 | 0 | 14 | 204 | 0.93 | 0.00 | 0.00 | 0.07 | A | 23 | 0.93 |
| 241 | 9 | 1 | 17 | 0 | 27 | 0.33 | 0.04 | 0.63 | 0.00 | G | 5 | 0.63 |
| 242 | 11 | 51 | 16 | 21 | 99 | 0.11 | 0.52 | 0.16 | 0.21 | T | 12 | 0.52 |
| 243 | 33 | 41 | 20 | 0 | 94 | 0.35 | 0.44 | 0.21 | 0.00 | T | 10 | 0.44 |
| 244 | 173 | 35 | 20 | 10 | 238 | 0.73 | 0.15 | 0.08 | 0.04 | A | 38 | 0.73 |
| 245 | 67 | 1 | 9 | 47 | 124 | 0.54 | 0.01 | 0.07 | 0.38 | A | 18 | 0.54 |
| 246 | 25 | 52 | 0 | 8 | 85 | 0.29 | 0.61 | 0.00 | 0.09 | T | 20 | 0.61 |
| 247 | 29 | 39 | 23 | 14 | 105 | 0.28 | 0.37 | 0.22 | 0.13 | N | 24 | 0.37 |
| 248 | 34 | 40 | 0 | 16 | 90 | 0.38 | 0.44 | 0.00 | 0.18 | T | 17 | 0.44 |
| 249 | 1 | 1 | 26 | 12 | 40 | 0.03 | 0.03 | 0.65 | 0.30 | G | 13 | 0.65 |
| 250 | 32 | 22 | 16 | 0 | 70 | 0.46 | 0.31 | 0.23 | 0.00 | A | 9 | 0.46 |
| 251 | 11 | 13 | 18 | 9 | 51 | 0.22 | 0.25 | 0.35 | 0.18 | N | 8 | 0.35 |
| 252 | 11 | 17 | 6 | 25 | 59 | 0.19 | 0.29 | 0.10 | 0.42 | C | 6 | 0.42 |
| 253 | 9 | 4 | 25 | 25 | 63 | 0.14 | 0.06 | 0.40 | 0.40 | C | 12 | 0.40 |
| 254 | 0 | 14 | 13 | 13 | 40 | 0.00 | 0.35 | 0.32 | 0.32 | T | 10 | 0.35 |
| 255 | 38 | 0 | 8 | 13 | 59 | 0.64 | 0.00 | 0.14 | 0.22 | A | 13 | 0.64 |
| 256 | 36 | 2 | 10 | 28 | 76 | 0.47 | 0.03 | 0.13 | 0.37 | A | 10 | 0.47 |
| 257 | 13 | 0 | 19 | 21 | 53 | 0.25 | 0.00 | 0.36 | 0.40 | C | 5 | 0.40 |
| 258 | 27 | 60 | 0 | 7 | 94 | 0.29 | 0.64 | 0.00 | 0.07 | T | 11 | 0.64 |
| 259 | 48 | 0 | 23 | 30 | 101 | 0.48 | 0.00 | 0.23 | 0.30 | A | 11 | 0.48 |
| 260 | 46 | 50 | 10 | 16 | 122 | 0.38 | 0.41 | 0.08 | 0.13 | T | 13 | 0.41 |
| 261 | 32 | 0 | 0 | 36 | 68 | 0.47 | 0.00 | 0.00 | 0.53 | C | 11 | 0.53 |
| 262 | 0 | 0 | 41 | 33 | 74 | 0.00 | 0.00 | 0.55 | 0.45 | G | 8 | 0.55 |
| 263 | 23 | 36 | 0 | 56 | 115 | 0.20 | 0.31 | 0.00 | 0.49 | C | 11 | 0.49 |
| 264 | 0 | 30 | 31 | 0 | 61 | 0.00 | 0.49 | 0.51 | 0.00 | G | 10 | 0.51 |
| 265 | 43 | 4 | 15 | 82 | 144 | 0.30 | 0.03 | 0.10 | 0.57 | C | 27 | 0.57 |
| 266 | 25 | 16 | 27 | 0 | 68 | 0.37 | 0.24 | 0.40 | 0.00 | G | 12 | 0.40 |
| 267 | 23 | 0 | 25 | 19 | 67 | 0.34 | 0.00 | 0.37 | 0.28 | G | 10 | 0.37 |
| 268 | 26 | 14 | 29 | 0 | 69 | 0.38 | 0.20 | 0.42 | 0.00 | G | 16 | 0.42 |
| 269 | 24 | 30 | 12 | 9 | 75 | 0.32 | 0.40 | 0.16 | 0.12 | N | 10 | 0.40 |
| 270 | 33 | 0 | 22 | 18 | 73 | 0.45 | 0.00 | 0.30 | 0.25 | A | 10 | 0.45 |
| 271 | 5 | 15 | 22 | 19 | 61 | 0.08 | 0.25 | 0.36 | 0.31 | G | 9 | 0.36 |
| 272 | 15 | 24 | 9 | 0 | 48 | 0.31 | 0.50 | 0.19 | 0.00 | T | 9 | 0.50 |
| 273 | 20 | 47 | 12 | 5 | 84 | 0.24 | 0.56 | 0.14 | 0.06 | T | 13 | 0.56 |
| 274 | 46 | 42 | 1 | 2 | 91 | 0.51 | 0.46 | 0.01 | 0.02 | A | 13 | 0.51 |
| 275 | 33 | 35 | 26 | 5 | 99 | 0.33 | 0.35 | 0.26 | 0.05 | T | 9 | 0.35 |
| 276 | 50 | 0 | 10 | 40 | 100 | 0.50 | 0.00 | 0.10 | 0.40 | A | 13 | 0.50 |
| 277 | 36 | 48 | 26 | 7 | 117 | 0.31 | 0.41 | 0.22 | 0.06 | T | 11 | 0.41 |
| 278 | 38 | 14 | 57 | 10 | 119 | 0.32 | 0.12 | 0.48 | 0.08 | G | 9 | 0.48 |
| 279 | 79 | 9 | 50 | 0 | 138 | 0.57 | 0.07 | 0.36 | 0.00 | A | 18 | 0.57 |
| 280 | 68 | 30 | 53 | 35 | 186 | 0.37 | 0.16 | 0.28 | 0.19 | N | 8 | 0.37 |
| 281 | 54 | 24 | 70 | 52 | 200 | 0.27 | 0.12 | 0.35 | 0.26 | N | 13 | 0.35 |
| 282 | 67 | 27 | 58 | 11 | 163 | 0.41 | 0.17 | 0.36 | 0.07 | A | 16 | 0.41 |
| 283 | 37 | 21 | 38 | 23 | 119 | 0.31 | 0.18 | 0.32 | 0.19 | N | 12 | 0.32 |
| 284 | 24 | 32 | 27 | 5 | 88 | 0.27 | 0.36 | 0.31 | 0.06 | T | 9 | 0.36 |
| 285 | 0 | 13 | 22 | 44 | 79 | 0.00 | 0.16 | 0.28 | 0.56 | C | 12 | 0.56 |
| 286 | 8 | 39 | 17 | 17 | 81 | 0.10 | 0.48 | 0.21 | 0.21 | T | 16 | 0.48 |
| 287 | 29 | 7 | 9 | 12 | 57 | 0.51 | 0.12 | 0.16 | 0.21 | A | 10 | 0.51 |
| 288 | 7 | 36 | 8 | 0 | 51 | 0.14 | 0.71 | 0.16 | 0.00 | T | 18 | 0.71 |
| 289 | 9 | 26 | 10 | 11 | 56 | 0.16 | 0.46 | 0.18 | 0.20 | N | 12 | 0.46 |
| 290 | 10 | 72 | 8 | 3 | 93 | 0.11 | 0.77 | 0.09 | 0.03 | T | 27 | 0.77 |
| 291 | 11 | 63 | 12 | 0 | 86 | 0.13 | 0.73 | 0.14 | 0.00 | T | 34 | 0.73 |
| 292 | 50 | 1 | 25 | 0 | 76 | 0.66 | 0.01 | 0.33 | 0.00 | A | 27 | 0.66 |
| 293 | 17 | 20 | 15 | 25 | 77 | 0.22 | 0.26 | 0.19 | 0.32 | N | 11 | 0.32 |
| 294 | 0 | 3 | 19 | 11 | 33 | 0.00 | 0.09 | 0.58 | 0.33 | G | 6 | 0.58 |
| 295 | 27 | 0 | 25 | 0 | 52 | 0.52 | 0.00 | 0.48 | 0.00 | A | 18 | 0.52 |
| 296 | 10 | 49 | 25 | 14 | 98 | 0.10 | 0.50 | 0.26 | 0.14 | T | 28 | 0.50 |
| 297 | 30 | 10 | 22 | 1 | 63 | 0.48 | 0.16 | 0.35 | 0.02 | A | 10 | 0.48 |
| 298 | 14 | 0 | 19 | 25 | 58 | 0.24 | 0.00 | 0.33 | 0.43 | C | 13 | 0.43 |
| 299 | 16 | 25 | 1 | 17 | 59 | 0.27 | 0.42 | 0.02 | 0.29 | T | 10 | 0.42 |
| 300 | 29 | 0 | 3 | 0 | 32 | 0.91 | 0.00 | 0.09 | 0.00 | A | 13 | 0.91 |
| 301 | 18 | 47 | 10 | 0 | 75 | 0.24 | 0.63 | 0.13 | 0.00 | T | 23 | 0.63 |
| 302 | 27 | 0 | 1 | 42 | 70 | 0.39 | 0.00 | 0.01 | 0.60 | C | 17 | 0.60 |
| 303 | 23 | 18 | 11 | 39 | 91 | 0.25 | 0.20 | 0.12 | 0.43 | N | 27 | 0.43 |
| 304 | 4 | 18 | 4 | 37 | 63 | 0.06 | 0.29 | 0.06 | 0.59 | C | 15 | 0.59 |
| 305 | 0 | 50 | 6 | 0 | 56 | 0.00 | 0.89 | 0.11 | 0.00 | T | 23 | 0.89 |
| 306 | 9 | 0 | 14 | 17 | 40 | 0.22 | 0.00 | 0.35 | 0.42 | C | 8 | 0.42 |
| 307 | 27 | 25 | 8 | 9 | 69 | 0.39 | 0.36 | 0.12 | 0.13 | N | 11 | 0.39 |
| 308 | 0 | 0 | 11 | 32 | 43 | 0.00 | 0.00 | 0.26 | 0.74 | C | 13 | 0.74 |
| 309 | 27 | 60 | 0 | 32 | 119 | 0.23 | 0.50 | 0.00 | 0.27 | T | 18 | 0.50 |
| 310 | 35 | 45 | 12 | 9 | 101 | 0.35 | 0.45 | 0.12 | 0.09 | T | 13 | 0.45 |
| 311 | 0 | 0 | 35 | 10 | 45 | 0.00 | 0.00 | 0.78 | 0.22 | G | 12 | 0.78 |
| 312 | 34 | 23 | 9 | 18 | 84 | 0.40 | 0.27 | 0.11 | 0.21 | N | 11 | 0.40 |
| 313 | 16 | 45 | 6 | 0 | 67 | 0.24 | 0.67 | 0.09 | 0.00 | T | 13 | 0.67 |
| 314 | 25 | 36 | 6 | 16 | 83 | 0.30 | 0.43 | 0.07 | 0.19 | T | 13 | 0.43 |
| 315 | 35 | 0 | 14 | 16 | 65 | 0.54 | 0.00 | 0.22 | 0.25 | A | 11 | 0.54 |
| 316 | 29 | 11 | 16 | 11 | 67 | 0.43 | 0.16 | 0.24 | 0.16 | N | 13 | 0.43 |
| 317 | 42 | 17 | 4 | 18 | 81 | 0.52 | 0.21 | 0.05 | 0.22 | A | 23 | 0.52 |
| 318 | 0 | 0 | 17 | 9 | 26 | 0.00 | 0.00 | 0.65 | 0.35 | G | 19 | 0.65 |
| 319 | 24 | 32 | 17 | 15 | 88 | 0.27 | 0.36 | 0.19 | 0.17 | N | 11 | 0.36 |
| 320 | 10 | 56 | 8 | 12 | 86 | 0.12 | 0.65 | 0.09 | 0.14 | T | 19 | 0.65 |
| 321 | 0 | 56 | 0 | 22 | 78 | 0.00 | 0.72 | 0.00 | 0.28 | T | 18 | 0.72 |
| 322 | 35 | 26 | 14 | 22 | 97 | 0.36 | 0.27 | 0.14 | 0.23 | N | 8 | 0.36 |
| 323 | 7 | 6 | 6 | 19 | 38 | 0.18 | 0.16 | 0.16 | 0.50 | N | 8 | 0.50 |
| 324 | 4 | 31 | 17 | 7 | 59 | 0.07 | 0.53 | 0.29 | 0.12 | T | 9 | 0.53 |
| 325 | 20 | 0 | 14 | 0 | 34 | 0.59 | 0.00 | 0.41 | 0.00 | A | 5 | 0.59 |
| 326 | 36 | 38 | 20 | 4 | 98 | 0.37 | 0.39 | 0.20 | 0.04 | T | 11 | 0.39 |
| 327 | 18 | 30 | 1 | 14 | 63 | 0.29 | 0.48 | 0.02 | 0.22 | T | 10 | 0.48 |
| 328 | 24 | 40 | 18 | 7 | 89 | 0.27 | 0.45 | 0.20 | 0.08 | T | 9 | 0.45 |
| 329 | 0 | 1 | 10 | 40 | 51 | 0.00 | 0.02 | 0.20 | 0.78 | C | 13 | 0.78 |
| 330 | 7 | 12 | 8 | 43 | 70 | 0.10 | 0.17 | 0.11 | 0.61 | C | 10 | 0.61 |
| 331 | 18 | 1 | 27 | 18 | 64 | 0.28 | 0.02 | 0.42 | 0.28 | G | 11 | 0.42 |
| 332 | 0 | 53 | 6 | 10 | 69 | 0.00 | 0.77 | 0.09 | 0.14 | T | 34 | 0.77 |
| 333 | 25 | 5 | 25 | 23 | 78 | 0.32 | 0.06 | 0.32 | 0.29 | A | 12 | 0.32 |
| 334 | 6 | 0 | 11 | 29 | 46 | 0.13 | 0.00 | 0.24 | 0.63 | C | 10 | 0.63 |
| 335 | 16 | 0 | 11 | 28 | 55 | 0.29 | 0.00 | 0.20 | 0.51 | C | 11 | 0.51 |
| 336 | 35 | 26 | 0 | 10 | 71 | 0.49 | 0.37 | 0.00 | 0.14 | A | 13 | 0.49 |
| 337 | 15 | 21 | 25 | 10 | 71 | 0.21 | 0.30 | 0.35 | 0.14 | N | 9 | 0.35 |
| 338 | 25 | 0 | 17 | 28 | 70 | 0.36 | 0.00 | 0.24 | 0.40 | C | 10 | 0.40 |
| 339 | 10 | 17 | 12 | 0 | 39 | 0.26 | 0.44 | 0.31 | 0.00 | T | 9 | 0.44 |
| 340 | 28 | 17 | 21 | 3 | 69 | 0.41 | 0.25 | 0.30 | 0.04 | A | 11 | 0.41 |
| 341 | 21 | 42 | 11 | 42 | 116 | 0.18 | 0.36 | 0.09 | 0.36 | N | 10 | 0.36 |
| 342 | 17 | 34 | 11 | 20 | 82 | 0.21 | 0.41 | 0.13 | 0.24 | N | 10 | 0.41 |
| 343 | 34 | 0 | 14 | 24 | 72 | 0.47 | 0.00 | 0.19 | 0.33 | A | 11 | 0.47 |
| 344 | 14 | 53 | 6 | 26 | 99 | 0.14 | 0.54 | 0.06 | 0.26 | T | 13 | 0.54 |
| 345 | 25 | 1 | 28 | 21 | 75 | 0.33 | 0.01 | 0.37 | 0.28 | G | 9 | 0.37 |
| 346 | 29 | 34 | 5 | 35 | 103 | 0.28 | 0.33 | 0.05 | 0.34 | C | 13 | 0.34 |
| 347 | 25 | 0 | 4 | 48 | 77 | 0.32 | 0.00 | 0.05 | 0.62 | C | 11 | 0.62 |
| 348 | 0 | 0 | 8 | 32 | 40 | 0.00 | 0.00 | 0.20 | 0.80 | C | 10 | 0.80 |
Trimming thresholds :
| Raw sequence information | Values |
|---|---|
| Raw sequence length : | 348 bp |
| Mean of raw sequence Phred quality scores : | 25.51 |
| Quality report - Trimmed sequence information | Values |
|---|---|
| Trimmed sequence length : | 127 bp |
| Trimmed sequence start position : | 24 |
| Trimmed sequence end position : | 151 |
| Mean of trimmed sequence Phred quality scores : | 35.43 |
Trimming thresholds :
| Raw sequence information | Values |
|---|---|
| Raw sequence length : | 348 bp |
| Mean of raw sequence primary peak ratios : | 0.69 |
| Percentage of non-mixed positions on raw sequence : | 42.82 % |
| Mixed peak report - Trimmed sequence information | Values |
|---|---|
| Trimmed sequence length : | 178 bp |
| Trimmed sequence start position : | 8 |
| Trimmed sequence end position : | 185 |
| Mean of trimmed sequence primary peak ratios : | 0.81 |
| Percentage of non-mixed positions on trimmed sequence : | 70.79 % |
Warning: Trimmed sequence from the sequencing result #1 was found with insufficient length or insufficient quality and will not be used.
| Final trimmed sequence information | Values |
|---|---|
| Length : | 128 bp |
| Start position : | 24 |
| End position : | 151 |
| Mean of Phred quality scores : | 35.45 |
| Mean of primary peak ratio : | 0.82 |
| Percentage of non-mixed positions : | 73.44 % |
Sequence of primary peaks:
Length: 228 bp
## CCAAATAAATTCCATAGCCCTCTAACCTTAATTACTATCCCTCTTATAGAGGGTTAATTACCGCCCCCCGTACCGCTAATTAACTAAAAATTCACCTACCGACCACAACCAATCAACAACGCGAACCCCTCCCCAAAACGAAACTAACGACCCGCCCACCCGACCTCGCATAAACGCGATAACCCTCTAACCTAAAATTACTAAACCCTTTAGTGAGGGTTAATTAAA
Open chromatogram file in a new tab
| Position | A peak | T peak | G peak | C peak | Total | A ratio | T ratio | G ratio | C ratio | Primary base | Quality score | Primary ratio |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 0 | 0 | 0 | 4 | 4 | 0.00 | 0.00 | 0.00 | 1.00 | C | 16 | 1.00 |
| 2 | 0 | 0 | 0 | 944 | 944 | 0.00 | 0.00 | 0.00 | 1.00 | C | 12 | 1.00 |
| 3 | 2114 | 0 | 0 | 1742 | 3856 | 0.55 | 0.00 | 0.00 | 0.45 | A | 11 | 0.55 |
| 4 | 1913 | 796 | 0 | 639 | 3348 | 0.57 | 0.24 | 0.00 | 0.19 | A | 2 | 0.57 |
| 5 | 950 | 0 | 0 | 0 | 950 | 1.00 | 0.00 | 0.00 | 0.00 | A | 17 | 1.00 |
| 6 | 0 | 1596 | 0 | 2 | 1598 | 0.00 | 1.00 | 0.00 | 0.00 | T | 8 | 1.00 |
| 7 | 2128 | 1296 | 0 | 0 | 3424 | 0.62 | 0.38 | 0.00 | 0.00 | A | 8 | 0.62 |
| 8 | 2118 | 0 | 0 | 499 | 2617 | 0.81 | 0.00 | 0.00 | 0.19 | A | 11 | 0.81 |
| 9 | 1814 | 0 | 0 | 0 | 1814 | 1.00 | 0.00 | 0.00 | 0.00 | A | 24 | 1.00 |
| 10 | 1 | 1119 | 0 | 0 | 1120 | 0.00 | 1.00 | 0.00 | 0.00 | T | 24 | 1.00 |
| 11 | 0 | 1687 | 0 | 0 | 1687 | 0.00 | 1.00 | 0.00 | 0.00 | T | 24 | 1.00 |
| 12 | 0 | 0 | 0 | 1583 | 1583 | 0.00 | 0.00 | 0.00 | 1.00 | C | 25 | 1.00 |
| 13 | 0 | 0 | 0 | 1121 | 1121 | 0.00 | 0.00 | 0.00 | 1.00 | C | 20 | 1.00 |
| 14 | 1135 | 0 | 265 | 1 | 1401 | 0.81 | 0.00 | 0.19 | 0.00 | A | 16 | 0.81 |
| 15 | 0 | 314 | 2 | 1 | 317 | 0.00 | 0.99 | 0.01 | 0.00 | T | 19 | 0.99 |
| 16 | 908 | 0 | 0 | 318 | 1226 | 0.74 | 0.00 | 0.00 | 0.26 | A | 8 | 0.74 |
| 17 | 0 | 0 | 378 | 0 | 378 | 0.00 | 0.00 | 1.00 | 0.00 | G | 8 | 1.00 |
| 18 | 1 | 0 | 0 | 1525 | 1526 | 0.00 | 0.00 | 0.00 | 1.00 | C | 15 | 1.00 |
| 19 | 0 | 0 | 0 | 1407 | 1407 | 0.00 | 0.00 | 0.00 | 1.00 | C | 23 | 1.00 |
| 20 | 0 | 0 | 0 | 1133 | 1133 | 0.00 | 0.00 | 0.00 | 1.00 | C | 29 | 1.00 |
| 21 | 0 | 1113 | 0 | 0 | 1113 | 0.00 | 1.00 | 0.00 | 0.00 | T | 30 | 1.00 |
| 22 | 499 | 0 | 0 | 952 | 1451 | 0.34 | 0.00 | 0.00 | 0.66 | C | 15 | 0.66 |
| 23 | 0 | 513 | 0 | 1 | 514 | 0.00 | 1.00 | 0.00 | 0.00 | T | 25 | 1.00 |
| 24 | 1416 | 1 | 0 | 0 | 1417 | 1.00 | 0.00 | 0.00 | 0.00 | A | 28 | 1.00 |
| 25 | 1075 | 0 | 0 | 0 | 1075 | 1.00 | 0.00 | 0.00 | 0.00 | A | 46 | 1.00 |
| 26 | 0 | 0 | 0 | 575 | 575 | 0.00 | 0.00 | 0.00 | 1.00 | C | 41 | 1.00 |
| 27 | 186 | 0 | 0 | 710 | 896 | 0.21 | 0.00 | 0.00 | 0.79 | C | 40 | 0.79 |
| 28 | 0 | 516 | 0 | 1 | 517 | 0.00 | 1.00 | 0.00 | 0.00 | T | 40 | 1.00 |
| 29 | 0 | 226 | 0 | 0 | 226 | 0.00 | 1.00 | 0.00 | 0.00 | T | 10 | 1.00 |
| 30 | 902 | 0 | 0 | 0 | 902 | 1.00 | 0.00 | 0.00 | 0.00 | A | 18 | 1.00 |
| 31 | 922 | 0 | 0 | 173 | 1095 | 0.84 | 0.00 | 0.00 | 0.16 | A | 45 | 0.84 |
| 32 | 0 | 648 | 0 | 0 | 648 | 0.00 | 1.00 | 0.00 | 0.00 | T | 48 | 1.00 |
| 33 | 0 | 1064 | 171 | 0 | 1235 | 0.00 | 0.86 | 0.14 | 0.00 | T | 49 | 0.86 |
| 34 | 1135 | 0 | 0 | 1 | 1136 | 1.00 | 0.00 | 0.00 | 0.00 | A | 49 | 1.00 |
| 35 | 0 | 1 | 0 | 666 | 667 | 0.00 | 0.00 | 0.00 | 1.00 | C | 44 | 1.00 |
| 36 | 0 | 519 | 0 | 0 | 519 | 0.00 | 1.00 | 0.00 | 0.00 | T | 25 | 1.00 |
| 37 | 781 | 0 | 0 | 352 | 1133 | 0.69 | 0.00 | 0.00 | 0.31 | A | 8 | 0.69 |
| 38 | 0 | 441 | 0 | 0 | 441 | 0.00 | 1.00 | 0.00 | 0.00 | T | 8 | 1.00 |
| 39 | 2 | 0 | 0 | 1264 | 1266 | 0.00 | 0.00 | 0.00 | 1.00 | C | 45 | 1.00 |
| 40 | 212 | 0 | 0 | 677 | 889 | 0.24 | 0.00 | 0.00 | 0.76 | C | 20 | 0.76 |
| 41 | 0 | 1 | 0 | 832 | 833 | 0.00 | 0.00 | 0.00 | 1.00 | C | 20 | 1.00 |
| 42 | 0 | 805 | 0 | 0 | 805 | 0.00 | 1.00 | 0.00 | 0.00 | T | 44 | 1.00 |
| 43 | 0 | 0 | 0 | 236 | 236 | 0.00 | 0.00 | 0.00 | 1.00 | C | 27 | 1.00 |
| 44 | 259 | 1197 | 0 | 0 | 1456 | 0.18 | 0.82 | 0.00 | 0.00 | T | 29 | 0.82 |
| 45 | 592 | 816 | 0 | 0 | 1408 | 0.42 | 0.58 | 0.00 | 0.00 | T | 24 | 0.58 |
| 46 | 1487 | 0 | 0 | 0 | 1487 | 1.00 | 0.00 | 0.00 | 0.00 | A | 25 | 1.00 |
| 47 | 0 | 390 | 336 | 0 | 726 | 0.00 | 0.54 | 0.46 | 0.00 | T | 6 | 0.54 |
| 48 | 594 | 558 | 2 | 0 | 1154 | 0.51 | 0.48 | 0.00 | 0.00 | A | 6 | 0.51 |
| 49 | 214 | 2 | 652 | 0 | 868 | 0.25 | 0.00 | 0.75 | 0.00 | G | 20 | 0.75 |
| 50 | 1051 | 0 | 0 | 0 | 1051 | 1.00 | 0.00 | 0.00 | 0.00 | A | 25 | 1.00 |
| 51 | 2 | 0 | 1060 | 676 | 1738 | 0.00 | 0.00 | 0.61 | 0.39 | G | 10 | 0.61 |
| 52 | 0 | 0 | 1385 | 413 | 1798 | 0.00 | 0.00 | 0.77 | 0.23 | G | 15 | 0.77 |
| 53 | 0 | 1 | 868 | 537 | 1406 | 0.00 | 0.00 | 0.62 | 0.38 | G | 11 | 0.62 |
| 54 | 0 | 742 | 0 | 540 | 1282 | 0.00 | 0.58 | 0.00 | 0.42 | T | 16 | 0.58 |
| 55 | 0 | 706 | 524 | 0 | 1230 | 0.00 | 0.57 | 0.43 | 0.00 | T | 16 | 0.57 |
| 56 | 1458 | 0 | 0 | 0 | 1458 | 1.00 | 0.00 | 0.00 | 0.00 | A | 30 | 1.00 |
| 57 | 1264 | 0 | 0 | 0 | 1264 | 1.00 | 0.00 | 0.00 | 0.00 | A | 36 | 1.00 |
| 58 | 358 | 895 | 0 | 0 | 1253 | 0.29 | 0.71 | 0.00 | 0.00 | T | 37 | 0.71 |
| 59 | 0 | 2461 | 0 | 3 | 2464 | 0.00 | 1.00 | 0.00 | 0.00 | T | 16 | 1.00 |
| 60 | 3929 | 6 | 0 | 376 | 4311 | 0.91 | 0.00 | 0.00 | 0.09 | A | 17 | 0.91 |
| 61 | 87 | 0 | 0 | 464 | 551 | 0.16 | 0.00 | 0.00 | 0.84 | C | 25 | 0.84 |
| 62 | 0 | 0 | 0 | 545 | 545 | 0.00 | 0.00 | 0.00 | 1.00 | C | 30 | 1.00 |
| 63 | 0 | 0 | 594 | 13 | 607 | 0.00 | 0.00 | 0.98 | 0.02 | G | 51 | 0.98 |
| 64 | 0 | 0 | 0 | 675 | 675 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 65 | 0 | 0 | 0 | 850 | 850 | 0.00 | 0.00 | 0.00 | 1.00 | C | 54 | 1.00 |
| 66 | 0 | 0 | 0 | 777 | 777 | 0.00 | 0.00 | 0.00 | 1.00 | C | 55 | 1.00 |
| 67 | 0 | 0 | 0 | 889 | 889 | 0.00 | 0.00 | 0.00 | 1.00 | C | 61 | 1.00 |
| 68 | 0 | 0 | 0 | 776 | 776 | 0.00 | 0.00 | 0.00 | 1.00 | C | 61 | 1.00 |
| 69 | 0 | 0 | 0 | 686 | 686 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 70 | 0 | 0 | 687 | 0 | 687 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 71 | 0 | 389 | 0 | 0 | 389 | 0.00 | 1.00 | 0.00 | 0.00 | T | 28 | 1.00 |
| 72 | 735 | 0 | 0 | 0 | 735 | 1.00 | 0.00 | 0.00 | 0.00 | A | 51 | 1.00 |
| 73 | 1 | 0 | 0 | 771 | 772 | 0.00 | 0.00 | 0.00 | 1.00 | C | 51 | 1.00 |
| 74 | 0 | 0 | 0 | 708 | 708 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 75 | 0 | 0 | 751 | 31 | 782 | 0.00 | 0.00 | 0.96 | 0.04 | G | 58 | 0.96 |
| 76 | 0 | 0 | 1 | 550 | 551 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 77 | 0 | 561 | 0 | 0 | 561 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 78 | 666 | 0 | 0 | 0 | 666 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 79 | 709 | 0 | 0 | 0 | 709 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 80 | 1 | 547 | 0 | 0 | 548 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 81 | 0 | 826 | 0 | 0 | 826 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 82 | 811 | 0 | 0 | 0 | 811 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 83 | 478 | 0 | 0 | 0 | 478 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 84 | 1 | 0 | 0 | 471 | 472 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 85 | 0 | 542 | 0 | 0 | 542 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 86 | 771 | 1 | 0 | 0 | 772 | 1.00 | 0.00 | 0.00 | 0.00 | A | 61 | 1.00 |
| 87 | 543 | 0 | 0 | 0 | 543 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 88 | 510 | 0 | 0 | 0 | 510 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 89 | 682 | 0 | 0 | 0 | 682 | 1.00 | 0.00 | 0.00 | 0.00 | A | 51 | 1.00 |
| 90 | 557 | 0 | 0 | 0 | 557 | 1.00 | 0.00 | 0.00 | 0.00 | A | 51 | 1.00 |
| 91 | 1 | 655 | 0 | 0 | 656 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 92 | 0 | 858 | 0 | 2 | 860 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 93 | 1 | 0 | 0 | 725 | 726 | 0.00 | 0.00 | 0.00 | 1.00 | C | 51 | 1.00 |
| 94 | 372 | 0 | 0 | 22 | 394 | 0.94 | 0.00 | 0.00 | 0.06 | A | 51 | 0.94 |
| 95 | 0 | 0 | 0 | 612 | 612 | 0.00 | 0.00 | 0.00 | 1.00 | C | 51 | 1.00 |
| 96 | 0 | 2 | 0 | 688 | 690 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 97 | 0 | 615 | 0 | 2 | 617 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 98 | 885 | 0 | 0 | 2 | 887 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 99 | 0 | 0 | 0 | 635 | 635 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 100 | 0 | 0 | 0 | 594 | 594 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 101 | 2 | 0 | 743 | 2 | 747 | 0.00 | 0.00 | 0.99 | 0.00 | G | 41 | 0.99 |
| 102 | 906 | 0 | 2 | 2 | 910 | 1.00 | 0.00 | 0.00 | 0.00 | A | 51 | 1.00 |
| 103 | 1 | 0 | 0 | 666 | 667 | 0.00 | 0.00 | 0.00 | 1.00 | C | 51 | 1.00 |
| 104 | 1 | 0 | 0 | 600 | 601 | 0.00 | 0.00 | 0.00 | 1.00 | C | 51 | 1.00 |
| 105 | 507 | 0 | 0 | 0 | 507 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 106 | 2 | 0 | 0 | 528 | 530 | 0.00 | 0.00 | 0.00 | 1.00 | C | 61 | 1.00 |
| 107 | 476 | 0 | 0 | 2 | 478 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 108 | 518 | 0 | 0 | 2 | 520 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 109 | 2 | 0 | 0 | 589 | 591 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 110 | 0 | 0 | 0 | 612 | 612 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 111 | 549 | 0 | 0 | 0 | 549 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 112 | 563 | 2 | 0 | 0 | 565 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 113 | 0 | 493 | 0 | 0 | 493 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 114 | 0 | 2 | 0 | 827 | 829 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 115 | 496 | 0 | 0 | 0 | 496 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 116 | 461 | 0 | 0 | 0 | 461 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 117 | 4 | 0 | 0 | 487 | 491 | 0.01 | 0.00 | 0.00 | 0.99 | C | 58 | 0.99 |
| 118 | 552 | 0 | 0 | 2 | 554 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 119 | 498 | 0 | 0 | 0 | 498 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 120 | 2 | 0 | 0 | 578 | 580 | 0.00 | 0.00 | 0.00 | 1.00 | C | 51 | 1.00 |
| 121 | 0 | 0 | 604 | 7 | 611 | 0.00 | 0.00 | 0.99 | 0.01 | G | 51 | 0.99 |
| 122 | 0 | 0 | 18 | 641 | 659 | 0.00 | 0.00 | 0.03 | 0.97 | C | 58 | 0.97 |
| 123 | 7 | 0 | 477 | 0 | 484 | 0.01 | 0.00 | 0.99 | 0.00 | G | 51 | 0.99 |
| 124 | 688 | 0 | 0 | 0 | 688 | 1.00 | 0.00 | 0.00 | 0.00 | A | 46 | 1.00 |
| 125 | 871 | 0 | 0 | 2 | 873 | 1.00 | 0.00 | 0.00 | 0.00 | A | 51 | 1.00 |
| 126 | 1 | 0 | 0 | 710 | 711 | 0.00 | 0.00 | 0.00 | 1.00 | C | 51 | 1.00 |
| 127 | 0 | 0 | 0 | 871 | 871 | 0.00 | 0.00 | 0.00 | 1.00 | C | 61 | 1.00 |
| 128 | 0 | 0 | 0 | 927 | 927 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 129 | 0 | 9 | 0 | 750 | 759 | 0.00 | 0.01 | 0.00 | 0.99 | C | 58 | 0.99 |
| 130 | 0 | 717 | 0 | 37 | 754 | 0.00 | 0.95 | 0.00 | 0.05 | T | 58 | 0.95 |
| 131 | 0 | 0 | 0 | 1034 | 1034 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 132 | 0 | 0 | 0 | 835 | 835 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 133 | 0 | 0 | 0 | 768 | 768 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 134 | 0 | 0 | 0 | 647 | 647 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 135 | 542 | 0 | 0 | 2 | 544 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 136 | 582 | 0 | 0 | 0 | 582 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 137 | 745 | 0 | 0 | 0 | 745 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 138 | 663 | 0 | 0 | 0 | 663 | 1.00 | 0.00 | 0.00 | 0.00 | A | 51 | 1.00 |
| 139 | 0 | 0 | 0 | 490 | 490 | 0.00 | 0.00 | 0.00 | 1.00 | C | 51 | 1.00 |
| 140 | 137 | 0 | 481 | 2 | 620 | 0.22 | 0.00 | 0.78 | 0.00 | G | 34 | 0.78 |
| 141 | 676 | 0 | 0 | 0 | 676 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 142 | 831 | 0 | 0 | 0 | 831 | 1.00 | 0.00 | 0.00 | 0.00 | A | 51 | 1.00 |
| 143 | 580 | 0 | 0 | 0 | 580 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 144 | 1 | 6 | 0 | 580 | 587 | 0.00 | 0.01 | 0.00 | 0.99 | C | 58 | 0.99 |
| 145 | 0 | 585 | 0 | 2 | 587 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 146 | 907 | 2 | 0 | 0 | 909 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 147 | 647 | 0 | 0 | 1 | 648 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 148 | 1 | 0 | 140 | 518 | 659 | 0.00 | 0.00 | 0.21 | 0.79 | C | 34 | 0.79 |
| 149 | 29 | 0 | 668 | 2 | 699 | 0.04 | 0.00 | 0.96 | 0.00 | G | 34 | 0.96 |
| 150 | 834 | 0 | 0 | 2 | 836 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 151 | 0 | 0 | 0 | 733 | 733 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 152 | 0 | 0 | 0 | 809 | 809 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 153 | 0 | 0 | 0 | 803 | 803 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 154 | 0 | 0 | 670 | 31 | 701 | 0.00 | 0.00 | 0.96 | 0.04 | G | 58 | 0.96 |
| 155 | 0 | 0 | 2 | 709 | 711 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 156 | 0 | 0 | 0 | 944 | 944 | 0.00 | 0.00 | 0.00 | 1.00 | C | 51 | 1.00 |
| 157 | 2 | 0 | 0 | 729 | 731 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 158 | 569 | 0 | 0 | 38 | 607 | 0.94 | 0.00 | 0.00 | 0.06 | A | 58 | 0.94 |
| 159 | 2 | 0 | 0 | 746 | 748 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 160 | 0 | 0 | 0 | 789 | 789 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 161 | 0 | 0 | 0 | 762 | 762 | 0.00 | 0.00 | 0.00 | 1.00 | C | 51 | 1.00 |
| 162 | 121 | 0 | 639 | 0 | 760 | 0.16 | 0.00 | 0.84 | 0.00 | G | 29 | 0.84 |
| 163 | 987 | 0 | 0 | 2 | 989 | 1.00 | 0.00 | 0.00 | 0.00 | A | 41 | 1.00 |
| 164 | 2 | 0 | 0 | 678 | 680 | 0.00 | 0.00 | 0.00 | 1.00 | C | 51 | 1.00 |
| 165 | 0 | 8 | 0 | 743 | 751 | 0.00 | 0.01 | 0.00 | 0.99 | C | 58 | 0.99 |
| 166 | 0 | 578 | 0 | 64 | 642 | 0.00 | 0.90 | 0.00 | 0.10 | T | 58 | 0.90 |
| 167 | 0 | 1 | 0 | 1007 | 1008 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 168 | 0 | 0 | 450 | 38 | 488 | 0.00 | 0.00 | 0.92 | 0.08 | G | 58 | 0.92 |
| 169 | 6 | 0 | 0 | 523 | 529 | 0.01 | 0.00 | 0.00 | 0.99 | C | 51 | 0.99 |
| 170 | 595 | 4 | 0 | 0 | 599 | 0.99 | 0.01 | 0.00 | 0.00 | A | 58 | 0.99 |
| 171 | 71 | 483 | 0 | 0 | 554 | 0.13 | 0.87 | 0.00 | 0.00 | T | 58 | 0.87 |
| 172 | 943 | 2 | 0 | 0 | 945 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 173 | 633 | 0 | 0 | 0 | 633 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 174 | 471 | 0 | 0 | 2 | 473 | 1.00 | 0.00 | 0.00 | 0.00 | A | 51 | 1.00 |
| 175 | 0 | 0 | 7 | 596 | 603 | 0.00 | 0.00 | 0.01 | 0.99 | C | 51 | 0.99 |
| 176 | 0 | 0 | 530 | 32 | 562 | 0.00 | 0.00 | 0.94 | 0.06 | G | 58 | 0.94 |
| 177 | 0 | 0 | 17 | 740 | 757 | 0.00 | 0.00 | 0.02 | 0.98 | C | 58 | 0.98 |
| 178 | 100 | 0 | 506 | 0 | 606 | 0.17 | 0.00 | 0.83 | 0.00 | G | 32 | 0.83 |
| 179 | 659 | 2 | 0 | 0 | 661 | 1.00 | 0.00 | 0.00 | 0.00 | A | 51 | 1.00 |
| 180 | 72 | 464 | 0 | 0 | 536 | 0.13 | 0.87 | 0.00 | 0.00 | T | 58 | 0.87 |
| 181 | 829 | 0 | 0 | 0 | 829 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 182 | 618 | 0 | 0 | 0 | 618 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 183 | 2 | 0 | 0 | 772 | 774 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 184 | 0 | 0 | 0 | 875 | 875 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 185 | 0 | 7 | 0 | 743 | 750 | 0.00 | 0.01 | 0.00 | 0.99 | C | 58 | 0.99 |
| 186 | 0 | 675 | 0 | 48 | 723 | 0.00 | 0.93 | 0.00 | 0.07 | T | 58 | 0.93 |
| 187 | 0 | 18 | 0 | 898 | 916 | 0.00 | 0.02 | 0.00 | 0.98 | C | 51 | 0.98 |
| 188 | 0 | 481 | 0 | 0 | 481 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 189 | 841 | 0 | 0 | 0 | 841 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 190 | 553 | 0 | 0 | 2 | 555 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 191 | 0 | 0 | 0 | 628 | 628 | 0.00 | 0.00 | 0.00 | 1.00 | C | 51 | 1.00 |
| 192 | 0 | 3 | 0 | 687 | 690 | 0.00 | 0.00 | 0.00 | 1.00 | C | 51 | 1.00 |
| 193 | 31 | 491 | 0 | 0 | 522 | 0.06 | 0.94 | 0.00 | 0.00 | T | 51 | 0.94 |
| 194 | 834 | 2 | 0 | 0 | 836 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 195 | 560 | 0 | 0 | 0 | 560 | 1.00 | 0.00 | 0.00 | 0.00 | A | 61 | 1.00 |
| 196 | 542 | 0 | 0 | 0 | 542 | 1.00 | 0.00 | 0.00 | 0.00 | A | 51 | 1.00 |
| 197 | 602 | 13 | 0 | 0 | 615 | 0.98 | 0.02 | 0.00 | 0.00 | A | 46 | 0.98 |
| 198 | 0 | 721 | 0 | 0 | 721 | 0.00 | 1.00 | 0.00 | 0.00 | T | 46 | 1.00 |
| 199 | 0 | 848 | 0 | 0 | 848 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 200 | 794 | 0 | 0 | 2 | 796 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 201 | 0 | 3 | 0 | 575 | 578 | 0.00 | 0.01 | 0.00 | 0.99 | C | 58 | 0.99 |
| 202 | 164 | 450 | 0 | 2 | 616 | 0.27 | 0.73 | 0.00 | 0.00 | T | 34 | 0.73 |
| 203 | 854 | 2 | 0 | 0 | 856 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 204 | 580 | 0 | 0 | 0 | 580 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 205 | 425 | 0 | 0 | 84 | 509 | 0.83 | 0.00 | 0.00 | 0.17 | A | 34 | 0.83 |
| 206 | 0 | 0 | 0 | 634 | 634 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 207 | 0 | 0 | 0 | 787 | 787 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 208 | 0 | 79 | 0 | 429 | 508 | 0.00 | 0.16 | 0.00 | 0.84 | C | 34 | 0.84 |
| 209 | 0 | 768 | 0 | 3 | 771 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 210 | 10 | 1012 | 0 | 0 | 1022 | 0.01 | 0.99 | 0.00 | 0.00 | T | 58 | 0.99 |
| 211 | 83 | 695 | 0 | 0 | 778 | 0.11 | 0.89 | 0.00 | 0.00 | T | 58 | 0.89 |
| 212 | 769 | 0 | 2 | 0 | 771 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 213 | 148 | 7 | 555 | 0 | 710 | 0.21 | 0.01 | 0.78 | 0.00 | G | 29 | 0.78 |
| 214 | 0 | 453 | 309 | 0 | 762 | 0.00 | 0.59 | 0.41 | 0.00 | T | 25 | 0.59 |
| 215 | 19 | 0 | 444 | 0 | 463 | 0.04 | 0.00 | 0.96 | 0.00 | G | 41 | 0.96 |
| 216 | 507 | 0 | 248 | 0 | 755 | 0.67 | 0.00 | 0.33 | 0.00 | A | 31 | 0.67 |
| 217 | 1 | 0 | 824 | 0 | 825 | 0.00 | 0.00 | 1.00 | 0.00 | G | 46 | 1.00 |
| 218 | 0 | 0 | 1023 | 0 | 1023 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 219 | 0 | 8 | 673 | 0 | 681 | 0.00 | 0.01 | 0.99 | 0.00 | G | 58 | 0.99 |
| 220 | 0 | 627 | 1 | 0 | 628 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 221 | 237 | 597 | 0 | 0 | 834 | 0.28 | 0.72 | 0.00 | 0.00 | T | 32 | 0.72 |
| 222 | 861 | 2 | 0 | 0 | 863 | 1.00 | 0.00 | 0.00 | 0.00 | A | 48 | 1.00 |
| 223 | 696 | 150 | 0 | 0 | 846 | 0.82 | 0.18 | 0.00 | 0.00 | A | 30 | 0.82 |
| 224 | 0 | 931 | 0 | 0 | 931 | 0.00 | 1.00 | 0.00 | 0.00 | T | 20 | 1.00 |
| 225 | 825 | 1834 | 0 | 0 | 2659 | 0.31 | 0.69 | 0.00 | 0.00 | T | 24 | 0.69 |
| 226 | 2995 | 8 | 0 | 0 | 3003 | 1.00 | 0.00 | 0.00 | 0.00 | A | 30 | 1.00 |
| 227 | 407 | 0 | 0 | 0 | 407 | 1.00 | 0.00 | 0.00 | 0.00 | A | 22 | 1.00 |
| 228 | 12 | 0 | 3 | 3 | 18 | 0.67 | 0.00 | 0.17 | 0.17 | A | 5 | 0.67 |
Trimming thresholds :
| Raw sequence information | Values |
|---|---|
| Raw sequence length : | 228 bp |
| Mean of raw sequence Phred quality scores : | 44.52 |
| Quality report - Trimmed sequence information | Values |
|---|---|
| Trimmed sequence length : | 197 bp |
| Trimmed sequence start position : | 25 |
| Trimmed sequence end position : | 222 |
| Mean of trimmed sequence Phred quality scores : | 48.5 |
Trimming thresholds :
| Raw sequence information | Values |
|---|---|
| Raw sequence length : | 228 bp |
| Mean of raw sequence primary peak ratios : | 0.95 |
| Percentage of non-mixed positions on raw sequence : | 91.23 % |
| Mixed peak report - Trimmed sequence information | Values |
|---|---|
| Trimmed sequence length : | 217 bp |
| Trimmed sequence start position : | 8 |
| Trimmed sequence end position : | 224 |
| Mean of trimmed sequence primary peak ratios : | 0.95 |
| Percentage of non-mixed positions on trimmed sequence : | 93.09 % |
| Final trimmed sequence information | Values |
|---|---|
| Length : | 198 bp |
| Start position : | 25 |
| End position : | 222 |
| Mean of Phred quality scores : | 48.49 |
| Mean of primary peak ratio : | 0.95 |
| Percentage of non-mixed positions : | 93.43 % |
Sequence of primary peaks:
## ACCTTAATTACTATCCCTCTTATAGAGGGTTAATTACCGCCCCCCGTACCGCTAATTAACTAAAAATTCACCTACCGACCACAACCAATCAACAACGCGAACCCCTCCCCAAAACGAAACTAACGACCCGCCCACCCGACCTCGCATAAACGCGATAACCCTCTAACCTAAAATTACTAAACCCTTTAGTGAGGGTTA
Open chromatogram file in a new tab
| Position | A peak | T peak | G peak | C peak | Total | A ratio | T ratio | G ratio | C ratio | Primary base | Quality score | Primary ratio |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 25 | 1075 | 0 | 0 | 0 | 1075 | 1.00 | 0.00 | 0.00 | 0.00 | A | 46 | 1.00 |
| 26 | 0 | 0 | 0 | 575 | 575 | 0.00 | 0.00 | 0.00 | 1.00 | C | 41 | 1.00 |
| 27 | 186 | 0 | 0 | 710 | 896 | 0.21 | 0.00 | 0.00 | 0.79 | C | 40 | 0.79 |
| 28 | 0 | 516 | 0 | 1 | 517 | 0.00 | 1.00 | 0.00 | 0.00 | T | 40 | 1.00 |
| 29 | 0 | 226 | 0 | 0 | 226 | 0.00 | 1.00 | 0.00 | 0.00 | T | 10 | 1.00 |
| 30 | 902 | 0 | 0 | 0 | 902 | 1.00 | 0.00 | 0.00 | 0.00 | A | 18 | 1.00 |
| 31 | 922 | 0 | 0 | 173 | 1095 | 0.84 | 0.00 | 0.00 | 0.16 | A | 45 | 0.84 |
| 32 | 0 | 648 | 0 | 0 | 648 | 0.00 | 1.00 | 0.00 | 0.00 | T | 48 | 1.00 |
| 33 | 0 | 1064 | 171 | 0 | 1235 | 0.00 | 0.86 | 0.14 | 0.00 | T | 49 | 0.86 |
| 34 | 1135 | 0 | 0 | 1 | 1136 | 1.00 | 0.00 | 0.00 | 0.00 | A | 49 | 1.00 |
| 35 | 0 | 1 | 0 | 666 | 667 | 0.00 | 0.00 | 0.00 | 1.00 | C | 44 | 1.00 |
| 36 | 0 | 519 | 0 | 0 | 519 | 0.00 | 1.00 | 0.00 | 0.00 | T | 25 | 1.00 |
| 37 | 781 | 0 | 0 | 352 | 1133 | 0.69 | 0.00 | 0.00 | 0.31 | A | 8 | 0.69 |
| 38 | 0 | 441 | 0 | 0 | 441 | 0.00 | 1.00 | 0.00 | 0.00 | T | 8 | 1.00 |
| 39 | 2 | 0 | 0 | 1264 | 1266 | 0.00 | 0.00 | 0.00 | 1.00 | C | 45 | 1.00 |
| 40 | 212 | 0 | 0 | 677 | 889 | 0.24 | 0.00 | 0.00 | 0.76 | C | 20 | 0.76 |
| 41 | 0 | 1 | 0 | 832 | 833 | 0.00 | 0.00 | 0.00 | 1.00 | C | 20 | 1.00 |
| 42 | 0 | 805 | 0 | 0 | 805 | 0.00 | 1.00 | 0.00 | 0.00 | T | 44 | 1.00 |
| 43 | 0 | 0 | 0 | 236 | 236 | 0.00 | 0.00 | 0.00 | 1.00 | C | 27 | 1.00 |
| 44 | 259 | 1197 | 0 | 0 | 1456 | 0.18 | 0.82 | 0.00 | 0.00 | T | 29 | 0.82 |
| 45 | 592 | 816 | 0 | 0 | 1408 | 0.42 | 0.58 | 0.00 | 0.00 | T | 24 | 0.58 |
| 46 | 1487 | 0 | 0 | 0 | 1487 | 1.00 | 0.00 | 0.00 | 0.00 | A | 25 | 1.00 |
| 47 | 0 | 390 | 336 | 0 | 726 | 0.00 | 0.54 | 0.46 | 0.00 | T | 6 | 0.54 |
| 48 | 594 | 558 | 2 | 0 | 1154 | 0.51 | 0.48 | 0.00 | 0.00 | A | 6 | 0.51 |
| 49 | 214 | 2 | 652 | 0 | 868 | 0.25 | 0.00 | 0.75 | 0.00 | G | 20 | 0.75 |
| 50 | 1051 | 0 | 0 | 0 | 1051 | 1.00 | 0.00 | 0.00 | 0.00 | A | 25 | 1.00 |
| 51 | 2 | 0 | 1060 | 676 | 1738 | 0.00 | 0.00 | 0.61 | 0.39 | G | 10 | 0.61 |
| 52 | 0 | 0 | 1385 | 413 | 1798 | 0.00 | 0.00 | 0.77 | 0.23 | G | 15 | 0.77 |
| 53 | 0 | 1 | 868 | 537 | 1406 | 0.00 | 0.00 | 0.62 | 0.38 | G | 11 | 0.62 |
| 54 | 0 | 742 | 0 | 540 | 1282 | 0.00 | 0.58 | 0.00 | 0.42 | T | 16 | 0.58 |
| 55 | 0 | 706 | 524 | 0 | 1230 | 0.00 | 0.57 | 0.43 | 0.00 | T | 16 | 0.57 |
| 56 | 1458 | 0 | 0 | 0 | 1458 | 1.00 | 0.00 | 0.00 | 0.00 | A | 30 | 1.00 |
| 57 | 1264 | 0 | 0 | 0 | 1264 | 1.00 | 0.00 | 0.00 | 0.00 | A | 36 | 1.00 |
| 58 | 358 | 895 | 0 | 0 | 1253 | 0.29 | 0.71 | 0.00 | 0.00 | T | 37 | 0.71 |
| 59 | 0 | 2461 | 0 | 3 | 2464 | 0.00 | 1.00 | 0.00 | 0.00 | T | 16 | 1.00 |
| 60 | 3929 | 6 | 0 | 376 | 4311 | 0.91 | 0.00 | 0.00 | 0.09 | A | 17 | 0.91 |
| 61 | 87 | 0 | 0 | 464 | 551 | 0.16 | 0.00 | 0.00 | 0.84 | C | 25 | 0.84 |
| 62 | 0 | 0 | 0 | 545 | 545 | 0.00 | 0.00 | 0.00 | 1.00 | C | 30 | 1.00 |
| 63 | 0 | 0 | 594 | 13 | 607 | 0.00 | 0.00 | 0.98 | 0.02 | G | 51 | 0.98 |
| 64 | 0 | 0 | 0 | 675 | 675 | 0.00 | 0.00 | 0.00 | 1.00 | C | 52 | 1.00 |
| 65 | 0 | 0 | 0 | 850 | 850 | 0.00 | 0.00 | 0.00 | 1.00 | C | 54 | 1.00 |
| 66 | 0 | 0 | 0 | 777 | 777 | 0.00 | 0.00 | 0.00 | 1.00 | C | 55 | 1.00 |
| 67 | 0 | 0 | 0 | 889 | 889 | 0.00 | 0.00 | 0.00 | 1.00 | C | 61 | 1.00 |
| 68 | 0 | 0 | 0 | 776 | 776 | 0.00 | 0.00 | 0.00 | 1.00 | C | 61 | 1.00 |
| 69 | 0 | 0 | 0 | 686 | 686 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 70 | 0 | 0 | 687 | 0 | 687 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 71 | 0 | 389 | 0 | 0 | 389 | 0.00 | 1.00 | 0.00 | 0.00 | T | 28 | 1.00 |
| 72 | 735 | 0 | 0 | 0 | 735 | 1.00 | 0.00 | 0.00 | 0.00 | A | 51 | 1.00 |
| 73 | 1 | 0 | 0 | 771 | 772 | 0.00 | 0.00 | 0.00 | 1.00 | C | 51 | 1.00 |
| 74 | 0 | 0 | 0 | 708 | 708 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 75 | 0 | 0 | 751 | 31 | 782 | 0.00 | 0.00 | 0.96 | 0.04 | G | 58 | 0.96 |
| 76 | 0 | 0 | 1 | 550 | 551 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 77 | 0 | 561 | 0 | 0 | 561 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 78 | 666 | 0 | 0 | 0 | 666 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 79 | 709 | 0 | 0 | 0 | 709 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 80 | 1 | 547 | 0 | 0 | 548 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 81 | 0 | 826 | 0 | 0 | 826 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 82 | 811 | 0 | 0 | 0 | 811 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 83 | 478 | 0 | 0 | 0 | 478 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 84 | 1 | 0 | 0 | 471 | 472 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 85 | 0 | 542 | 0 | 0 | 542 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 86 | 771 | 1 | 0 | 0 | 772 | 1.00 | 0.00 | 0.00 | 0.00 | A | 61 | 1.00 |
| 87 | 543 | 0 | 0 | 0 | 543 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 88 | 510 | 0 | 0 | 0 | 510 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 89 | 682 | 0 | 0 | 0 | 682 | 1.00 | 0.00 | 0.00 | 0.00 | A | 51 | 1.00 |
| 90 | 557 | 0 | 0 | 0 | 557 | 1.00 | 0.00 | 0.00 | 0.00 | A | 51 | 1.00 |
| 91 | 1 | 655 | 0 | 0 | 656 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 92 | 0 | 858 | 0 | 2 | 860 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 93 | 1 | 0 | 0 | 725 | 726 | 0.00 | 0.00 | 0.00 | 1.00 | C | 51 | 1.00 |
| 94 | 372 | 0 | 0 | 22 | 394 | 0.94 | 0.00 | 0.00 | 0.06 | A | 51 | 0.94 |
| 95 | 0 | 0 | 0 | 612 | 612 | 0.00 | 0.00 | 0.00 | 1.00 | C | 51 | 1.00 |
| 96 | 0 | 2 | 0 | 688 | 690 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 97 | 0 | 615 | 0 | 2 | 617 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 98 | 885 | 0 | 0 | 2 | 887 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 99 | 0 | 0 | 0 | 635 | 635 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 100 | 0 | 0 | 0 | 594 | 594 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 101 | 2 | 0 | 743 | 2 | 747 | 0.00 | 0.00 | 0.99 | 0.00 | G | 41 | 0.99 |
| 102 | 906 | 0 | 2 | 2 | 910 | 1.00 | 0.00 | 0.00 | 0.00 | A | 51 | 1.00 |
| 103 | 1 | 0 | 0 | 666 | 667 | 0.00 | 0.00 | 0.00 | 1.00 | C | 51 | 1.00 |
| 104 | 1 | 0 | 0 | 600 | 601 | 0.00 | 0.00 | 0.00 | 1.00 | C | 51 | 1.00 |
| 105 | 507 | 0 | 0 | 0 | 507 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 106 | 2 | 0 | 0 | 528 | 530 | 0.00 | 0.00 | 0.00 | 1.00 | C | 61 | 1.00 |
| 107 | 476 | 0 | 0 | 2 | 478 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 108 | 518 | 0 | 0 | 2 | 520 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 109 | 2 | 0 | 0 | 589 | 591 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 110 | 0 | 0 | 0 | 612 | 612 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 111 | 549 | 0 | 0 | 0 | 549 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 112 | 563 | 2 | 0 | 0 | 565 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 113 | 0 | 493 | 0 | 0 | 493 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 114 | 0 | 2 | 0 | 827 | 829 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 115 | 496 | 0 | 0 | 0 | 496 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 116 | 461 | 0 | 0 | 0 | 461 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 117 | 4 | 0 | 0 | 487 | 491 | 0.01 | 0.00 | 0.00 | 0.99 | C | 58 | 0.99 |
| 118 | 552 | 0 | 0 | 2 | 554 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 119 | 498 | 0 | 0 | 0 | 498 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 120 | 2 | 0 | 0 | 578 | 580 | 0.00 | 0.00 | 0.00 | 1.00 | C | 51 | 1.00 |
| 121 | 0 | 0 | 604 | 7 | 611 | 0.00 | 0.00 | 0.99 | 0.01 | G | 51 | 0.99 |
| 122 | 0 | 0 | 18 | 641 | 659 | 0.00 | 0.00 | 0.03 | 0.97 | C | 58 | 0.97 |
| 123 | 7 | 0 | 477 | 0 | 484 | 0.01 | 0.00 | 0.99 | 0.00 | G | 51 | 0.99 |
| 124 | 688 | 0 | 0 | 0 | 688 | 1.00 | 0.00 | 0.00 | 0.00 | A | 46 | 1.00 |
| 125 | 871 | 0 | 0 | 2 | 873 | 1.00 | 0.00 | 0.00 | 0.00 | A | 51 | 1.00 |
| 126 | 1 | 0 | 0 | 710 | 711 | 0.00 | 0.00 | 0.00 | 1.00 | C | 51 | 1.00 |
| 127 | 0 | 0 | 0 | 871 | 871 | 0.00 | 0.00 | 0.00 | 1.00 | C | 61 | 1.00 |
| 128 | 0 | 0 | 0 | 927 | 927 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 129 | 0 | 9 | 0 | 750 | 759 | 0.00 | 0.01 | 0.00 | 0.99 | C | 58 | 0.99 |
| 130 | 0 | 717 | 0 | 37 | 754 | 0.00 | 0.95 | 0.00 | 0.05 | T | 58 | 0.95 |
| 131 | 0 | 0 | 0 | 1034 | 1034 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 132 | 0 | 0 | 0 | 835 | 835 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 133 | 0 | 0 | 0 | 768 | 768 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 134 | 0 | 0 | 0 | 647 | 647 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 135 | 542 | 0 | 0 | 2 | 544 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 136 | 582 | 0 | 0 | 0 | 582 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 137 | 745 | 0 | 0 | 0 | 745 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 138 | 663 | 0 | 0 | 0 | 663 | 1.00 | 0.00 | 0.00 | 0.00 | A | 51 | 1.00 |
| 139 | 0 | 0 | 0 | 490 | 490 | 0.00 | 0.00 | 0.00 | 1.00 | C | 51 | 1.00 |
| 140 | 137 | 0 | 481 | 2 | 620 | 0.22 | 0.00 | 0.78 | 0.00 | G | 34 | 0.78 |
| 141 | 676 | 0 | 0 | 0 | 676 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 142 | 831 | 0 | 0 | 0 | 831 | 1.00 | 0.00 | 0.00 | 0.00 | A | 51 | 1.00 |
| 143 | 580 | 0 | 0 | 0 | 580 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 144 | 1 | 6 | 0 | 580 | 587 | 0.00 | 0.01 | 0.00 | 0.99 | C | 58 | 0.99 |
| 145 | 0 | 585 | 0 | 2 | 587 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 146 | 907 | 2 | 0 | 0 | 909 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 147 | 647 | 0 | 0 | 1 | 648 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 148 | 1 | 0 | 140 | 518 | 659 | 0.00 | 0.00 | 0.21 | 0.79 | C | 34 | 0.79 |
| 149 | 29 | 0 | 668 | 2 | 699 | 0.04 | 0.00 | 0.96 | 0.00 | G | 34 | 0.96 |
| 150 | 834 | 0 | 0 | 2 | 836 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 151 | 0 | 0 | 0 | 733 | 733 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 152 | 0 | 0 | 0 | 809 | 809 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 153 | 0 | 0 | 0 | 803 | 803 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 154 | 0 | 0 | 670 | 31 | 701 | 0.00 | 0.00 | 0.96 | 0.04 | G | 58 | 0.96 |
| 155 | 0 | 0 | 2 | 709 | 711 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 156 | 0 | 0 | 0 | 944 | 944 | 0.00 | 0.00 | 0.00 | 1.00 | C | 51 | 1.00 |
| 157 | 2 | 0 | 0 | 729 | 731 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 158 | 569 | 0 | 0 | 38 | 607 | 0.94 | 0.00 | 0.00 | 0.06 | A | 58 | 0.94 |
| 159 | 2 | 0 | 0 | 746 | 748 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 160 | 0 | 0 | 0 | 789 | 789 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 161 | 0 | 0 | 0 | 762 | 762 | 0.00 | 0.00 | 0.00 | 1.00 | C | 51 | 1.00 |
| 162 | 121 | 0 | 639 | 0 | 760 | 0.16 | 0.00 | 0.84 | 0.00 | G | 29 | 0.84 |
| 163 | 987 | 0 | 0 | 2 | 989 | 1.00 | 0.00 | 0.00 | 0.00 | A | 41 | 1.00 |
| 164 | 2 | 0 | 0 | 678 | 680 | 0.00 | 0.00 | 0.00 | 1.00 | C | 51 | 1.00 |
| 165 | 0 | 8 | 0 | 743 | 751 | 0.00 | 0.01 | 0.00 | 0.99 | C | 58 | 0.99 |
| 166 | 0 | 578 | 0 | 64 | 642 | 0.00 | 0.90 | 0.00 | 0.10 | T | 58 | 0.90 |
| 167 | 0 | 1 | 0 | 1007 | 1008 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 168 | 0 | 0 | 450 | 38 | 488 | 0.00 | 0.00 | 0.92 | 0.08 | G | 58 | 0.92 |
| 169 | 6 | 0 | 0 | 523 | 529 | 0.01 | 0.00 | 0.00 | 0.99 | C | 51 | 0.99 |
| 170 | 595 | 4 | 0 | 0 | 599 | 0.99 | 0.01 | 0.00 | 0.00 | A | 58 | 0.99 |
| 171 | 71 | 483 | 0 | 0 | 554 | 0.13 | 0.87 | 0.00 | 0.00 | T | 58 | 0.87 |
| 172 | 943 | 2 | 0 | 0 | 945 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 173 | 633 | 0 | 0 | 0 | 633 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 174 | 471 | 0 | 0 | 2 | 473 | 1.00 | 0.00 | 0.00 | 0.00 | A | 51 | 1.00 |
| 175 | 0 | 0 | 7 | 596 | 603 | 0.00 | 0.00 | 0.01 | 0.99 | C | 51 | 0.99 |
| 176 | 0 | 0 | 530 | 32 | 562 | 0.00 | 0.00 | 0.94 | 0.06 | G | 58 | 0.94 |
| 177 | 0 | 0 | 17 | 740 | 757 | 0.00 | 0.00 | 0.02 | 0.98 | C | 58 | 0.98 |
| 178 | 100 | 0 | 506 | 0 | 606 | 0.17 | 0.00 | 0.83 | 0.00 | G | 32 | 0.83 |
| 179 | 659 | 2 | 0 | 0 | 661 | 1.00 | 0.00 | 0.00 | 0.00 | A | 51 | 1.00 |
| 180 | 72 | 464 | 0 | 0 | 536 | 0.13 | 0.87 | 0.00 | 0.00 | T | 58 | 0.87 |
| 181 | 829 | 0 | 0 | 0 | 829 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 182 | 618 | 0 | 0 | 0 | 618 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 183 | 2 | 0 | 0 | 772 | 774 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 184 | 0 | 0 | 0 | 875 | 875 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 185 | 0 | 7 | 0 | 743 | 750 | 0.00 | 0.01 | 0.00 | 0.99 | C | 58 | 0.99 |
| 186 | 0 | 675 | 0 | 48 | 723 | 0.00 | 0.93 | 0.00 | 0.07 | T | 58 | 0.93 |
| 187 | 0 | 18 | 0 | 898 | 916 | 0.00 | 0.02 | 0.00 | 0.98 | C | 51 | 0.98 |
| 188 | 0 | 481 | 0 | 0 | 481 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 189 | 841 | 0 | 0 | 0 | 841 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 190 | 553 | 0 | 0 | 2 | 555 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 191 | 0 | 0 | 0 | 628 | 628 | 0.00 | 0.00 | 0.00 | 1.00 | C | 51 | 1.00 |
| 192 | 0 | 3 | 0 | 687 | 690 | 0.00 | 0.00 | 0.00 | 1.00 | C | 51 | 1.00 |
| 193 | 31 | 491 | 0 | 0 | 522 | 0.06 | 0.94 | 0.00 | 0.00 | T | 51 | 0.94 |
| 194 | 834 | 2 | 0 | 0 | 836 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 195 | 560 | 0 | 0 | 0 | 560 | 1.00 | 0.00 | 0.00 | 0.00 | A | 61 | 1.00 |
| 196 | 542 | 0 | 0 | 0 | 542 | 1.00 | 0.00 | 0.00 | 0.00 | A | 51 | 1.00 |
| 197 | 602 | 13 | 0 | 0 | 615 | 0.98 | 0.02 | 0.00 | 0.00 | A | 46 | 0.98 |
| 198 | 0 | 721 | 0 | 0 | 721 | 0.00 | 1.00 | 0.00 | 0.00 | T | 46 | 1.00 |
| 199 | 0 | 848 | 0 | 0 | 848 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 200 | 794 | 0 | 0 | 2 | 796 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 201 | 0 | 3 | 0 | 575 | 578 | 0.00 | 0.01 | 0.00 | 0.99 | C | 58 | 0.99 |
| 202 | 164 | 450 | 0 | 2 | 616 | 0.27 | 0.73 | 0.00 | 0.00 | T | 34 | 0.73 |
| 203 | 854 | 2 | 0 | 0 | 856 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 204 | 580 | 0 | 0 | 0 | 580 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 205 | 425 | 0 | 0 | 84 | 509 | 0.83 | 0.00 | 0.00 | 0.17 | A | 34 | 0.83 |
| 206 | 0 | 0 | 0 | 634 | 634 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 207 | 0 | 0 | 0 | 787 | 787 | 0.00 | 0.00 | 0.00 | 1.00 | C | 58 | 1.00 |
| 208 | 0 | 79 | 0 | 429 | 508 | 0.00 | 0.16 | 0.00 | 0.84 | C | 34 | 0.84 |
| 209 | 0 | 768 | 0 | 3 | 771 | 0.00 | 1.00 | 0.00 | 0.00 | T | 58 | 1.00 |
| 210 | 10 | 1012 | 0 | 0 | 1022 | 0.01 | 0.99 | 0.00 | 0.00 | T | 58 | 0.99 |
| 211 | 83 | 695 | 0 | 0 | 778 | 0.11 | 0.89 | 0.00 | 0.00 | T | 58 | 0.89 |
| 212 | 769 | 0 | 2 | 0 | 771 | 1.00 | 0.00 | 0.00 | 0.00 | A | 58 | 1.00 |
| 213 | 148 | 7 | 555 | 0 | 710 | 0.21 | 0.01 | 0.78 | 0.00 | G | 29 | 0.78 |
| 214 | 0 | 453 | 309 | 0 | 762 | 0.00 | 0.59 | 0.41 | 0.00 | T | 25 | 0.59 |
| 215 | 19 | 0 | 444 | 0 | 463 | 0.04 | 0.00 | 0.96 | 0.00 | G | 41 | 0.96 |
| 216 | 507 | 0 | 248 | 0 | 755 | 0.67 | 0.00 | 0.33 | 0.00 | A | 31 | 0.67 |
| 217 | 1 | 0 | 824 | 0 | 825 | 0.00 | 0.00 | 1.00 | 0.00 | G | 46 | 1.00 |
| 218 | 0 | 0 | 1023 | 0 | 1023 | 0.00 | 0.00 | 1.00 | 0.00 | G | 58 | 1.00 |
| 219 | 0 | 8 | 673 | 0 | 681 | 0.00 | 0.01 | 0.99 | 0.00 | G | 58 | 0.99 |
| 220 | 0 | 627 | 1 | 0 | 628 | 0.00 | 1.00 | 0.00 | 0.00 | T | 51 | 1.00 |
| 221 | 237 | 597 | 0 | 0 | 834 | 0.28 | 0.72 | 0.00 | 0.00 | T | 32 | 0.72 |
| 222 | 861 | 2 | 0 | 0 | 863 | 1.00 | 0.00 | 0.00 | 0.00 | A | 48 | 1.00 |
Sequencing result #1 is aligned on both sense and antisense bisulfite converted DNA sequences to determine the sequencing direction as forward or reverse.
Warning: Trimmed sequence from the sequencing result #1 was found with insufficient length or insufficient quality and will not be used.
Trimmed sequence of primary peaks from sequencing result #1 is aligned with bisulfite converted reference DNA sense sequence
Trimmed sequence of primary peaks from sequencing result #1 is aligned with bisulfite converted reference DNA antisense sequence
Warning: Insufficient quality of trimmed sequence for direction determination.
Sequencing result #2 is aligned on both sense and antisense bisulfite converted DNA sequences to determine the sequencing direction as forward or reverse.
Trimmed sequence of primary peaks from sequencing result #2 is aligned with bisulfite converted reference DNA sense sequence
## ########################################
## # Program: Biostrings (version 2.64.0), a Bioconductor package
## # Rundate: Sat Jun 4 11:17:48 2022
## ########################################
## #=======================================
## #
## # Aligned_sequences: 2
## # 1: P1
## # 2: S1
## # Matrix: NA
## # Gap_penalty: 14.0
## # Extend_penalty: 4.0
## #
## # Length: 10
## # Identity: 10/10 (100.0%)
## # Similarity: NA/10 (NA%)
## # Gaps: 0/10 (0.0%)
## # Score: 19.81756
## #
## #
## #=======================================
##
## P1 23 TAGAGGGTTA 32
## ||||||||||
## S1 85 TAGAGGGTTA 94
##
##
## #---------------------------------------
## #---------------------------------------
Trimmed sequence of primary peaks from sequencing result #2 is aligned with bisulfite converted reference DNA antisense sequence
## ########################################
## # Program: Biostrings (version 2.64.0), a Bioconductor package
## # Rundate: Sat Jun 4 11:17:48 2022
## ########################################
## #=======================================
## #
## # Aligned_sequences: 2
## # 1: P1
## # 2: S1
## # Matrix: NA
## # Gap_penalty: 14.0
## # Extend_penalty: 4.0
## #
## # Length: 146
## # Identity: 146/146 (100.0%)
## # Similarity: NA/146 (NA%)
## # Gaps: 0/146 (0.0%)
## # Score: 289.336
## #
## #
## #=======================================
##
## P1 36 ACCGCCCCCCGTACCGCTAA 55
## ||||||||||||||||||||
## S1 128 ACCGCCCCCCGTACCGCTAA 147
##
## P1 56 TTAACTAAAAATTCACCTAC 75
## ||||||||||||||||||||
## S1 148 TTAACTAAAAATTCACCTAC 167
##
## P1 76 CGACCACAACCAATCAACAA 95
## ||||||||||||||||||||
## S1 168 CGACCACAACCAATCAACAA 187
##
## P1 96 CGCGAACCCCTCCCCAAAAC 115
## ||||||||||||||||||||
## S1 188 CGCGAACCCCTCCCCAAAAC 207
##
## P1 116 GAAACTAACGACCCGCCCAC 135
## ||||||||||||||||||||
## S1 208 GAAACTAACGACCCGCCCAC 227
##
## P1 136 CCGACCTCGCATAAACGCGA 155
## ||||||||||||||||||||
## S1 228 CCGACCTCGCATAAACGCGA 247
##
## P1 156 TAACCCTCTAACCTAAAATT 175
## ||||||||||||||||||||
## S1 248 TAACCCTCTAACCTAAAATT 267
##
## P1 176 ACTAAA 181
## ||||||
## S1 268 ACTAAA 273
##
##
## #---------------------------------------
## #---------------------------------------
Sequencing result #2 corresponds to reverse direction
To find nucleotides of reference DNA sequence positions on sequencing result, the trimmed sequence of primary peaks from forward sequencing results is aligned with bisulfite converted reference DNA sense sequence.
Warning: Insufficient length or quality of trimmed forward sequencing.
Warning: Insufficient length or quality of trimmed forward sequencing.
To find nucleotides of reference DNA sequence positions on sequencing result, the trimmed sequence of primary peaks from reverse sequencing results is aligned with bisulfite converted reference DNA antisense sequence.
## ########################################
## # Program: Biostrings (version 2.64.0), a Bioconductor package
## # Rundate: Sat Jun 4 11:17:49 2022
## ########################################
## #=======================================
## #
## # Aligned_sequences: 2
## # 1: P1
## # 2: S1
## # Matrix: NA
## # Gap_penalty: 14.0
## # Extend_penalty: 4.0
## #
## # Length: 146
## # Identity: 146/146 (100.0%)
## # Similarity: NA/146 (NA%)
## # Gaps: 0/146 (0.0%)
## # Score: 289.336
## #
## #
## #=======================================
##
## P1 36 ACCGCCCCCCGTACCGCTAA 55
## ||||||||||||||||||||
## S1 128 ACCGCCCCCCGTACCGCTAA 147
##
## P1 56 TTAACTAAAAATTCACCTAC 75
## ||||||||||||||||||||
## S1 148 TTAACTAAAAATTCACCTAC 167
##
## P1 76 CGACCACAACCAATCAACAA 95
## ||||||||||||||||||||
## S1 168 CGACCACAACCAATCAACAA 187
##
## P1 96 CGCGAACCCCTCCCCAAAAC 115
## ||||||||||||||||||||
## S1 188 CGCGAACCCCTCCCCAAAAC 207
##
## P1 116 GAAACTAACGACCCGCCCAC 135
## ||||||||||||||||||||
## S1 208 GAAACTAACGACCCGCCCAC 227
##
## P1 136 CCGACCTCGCATAAACGCGA 155
## ||||||||||||||||||||
## S1 228 CCGACCTCGCATAAACGCGA 247
##
## P1 156 TAACCCTCTAACCTAAAATT 175
## ||||||||||||||||||||
## S1 248 TAACCCTCTAACCTAAAATT 267
##
## P1 176 ACTAAA 181
## ||||||
## S1 268 ACTAAA 273
##
##
## #---------------------------------------
## #---------------------------------------
Aligned sequence from reverse sequencing result (Pattern P1):
Length: 146 bp
## ACCGCCCCCCGTACCGCTAATTAACTAAAAATTCACCTACCGACCACAACCAATCAACAACGCGAACCCCTCCCCAAAACGAAACTAACGACCCGCCCACCCGACCTCGCATAAACGCGATAACCCTCTAACCTAAAATTACTAAA
Aligned sequence from bisulfite converted reference DNA antisense sequence (Subject S1):
Length: 146 bp
## ACCGCCCCCCGTACCGCTAATTAACTAAAAATTCACCTACCGACCACAACCAATCAACAACGCGAACCCCTCCCCAAAACGAAACTAACGACCCGCCCACCCGACCTCGCATAAACGCGATAACCCTCTAACCTAAAATTACTAAA
| Thresholds | Values |
|---|---|
| Minimum length of the aligned sequence : | 30 bp |
| Minimum identity percentage of alignment : | 75 % |
| Minimum of bisulfite conversion rate mean : | 0.9 |
| Direction | Sequencing | Length | Identity | Mismatch | Indel in DNA reference | Indel in sequencing | Mean of conversion rates | SD of conversion rates | Quality Result |
|---|---|---|---|---|---|---|---|---|---|
| Reverse | #2 | 146 | 100 | 0 | 0 | 0 | 1 | 0 | Correct |
Forward sequencing result could have not been trimmed due to poor quality.
No mismatch positions in reverse alignment.
No insertions/deletions in reverse alignment.
| C number | Bisulfite conversion rate Reverse |
|---|---|
| 50 | 1.00 |
| 49 | 1.00 |
| 48 | 1.00 |
| 47 | 1.00 |
| 46 | 1.00 |
| 45 | 1.00 |
| 44 | 1.00 |
| 43 | 1.00 |
| 42 | 1.00 |
| 41 | 1.00 |
| 40 | 1.00 |
| 39 | 1.00 |
| 38 | 1.00 |
| 37 | 1.00 |
| 36 | 1.00 |
| 35 | 1.00 |
| 34 | 1.00 |
| 33 | 1.00 |
| 32 | 1.00 |
| 31 | 1.00 |
| 30 | 1.00 |
| 29 | 1.00 |
| 28 | 1.00 |
| 27 | 1.00 |
| 26 | 1.00 |
| 25 | 1.00 |
| 24 | 1.00 |
| 23 | 1.00 |
| 22 | 1.00 |
| 21 | 1.00 |
| 20 | 1.00 |
| 19 | NaN |
| C number | Position in reference DNA | Position in sequencing result (trimmed) | Position in sequencing result (raw) | A peak | T peak | G peak | C peak | Bisulfite conversion rate |
|---|---|---|---|---|---|---|---|---|
| 50 | 146 | 54 | 78 | 666 | 0 | 0 | 0 | 1 |
| 49 | 150 | 58 | 82 | 811 | 0 | 0 | 0 | 1 |
| 48 | 151 | 59 | 83 | 478 | 0 | 0 | 0 | 1 |
| 47 | 154 | 62 | 86 | 771 | 1 | 0 | 0 | 1 |
| 46 | 156 | 64 | 88 | 510 | 0 | 0 | 0 | 1 |
| 45 | 157 | 65 | 89 | 682 | 0 | 0 | 0 | 1 |
| 44 | 158 | 66 | 90 | 557 | 0 | 0 | 0 | 1 |
| 43 | 166 | 74 | 98 | 885 | 0 | 0 | 2 | 1 |
| 42 | 170 | 78 | 102 | 906 | 0 | 2 | 2 | 1 |
| 41 | 176 | 84 | 108 | 518 | 0 | 0 | 2 | 1 |
| 40 | 184 | 92 | 116 | 461 | 0 | 0 | 0 | 1 |
| 39 | 187 | 95 | 119 | 498 | 0 | 0 | 0 | 1 |
| 38 | 192 | 100 | 124 | 688 | 0 | 0 | 0 | 1 |
| 37 | 204 | 112 | 136 | 582 | 0 | 0 | 0 | 1 |
| 36 | 205 | 113 | 137 | 745 | 0 | 0 | 0 | 1 |
| 35 | 206 | 114 | 138 | 663 | 0 | 0 | 0 | 1 |
| 34 | 209 | 117 | 141 | 676 | 0 | 0 | 0 | 1 |
| 33 | 211 | 119 | 143 | 580 | 0 | 0 | 0 | 1 |
| 32 | 214 | 122 | 146 | 907 | 2 | 0 | 0 | 1 |
| 31 | 218 | 126 | 150 | 834 | 0 | 0 | 2 | 1 |
| 30 | 231 | 139 | 163 | 987 | 0 | 0 | 2 | 1 |
| 29 | 241 | 149 | 173 | 633 | 0 | 0 | 0 | 1 |
| 28 | 247 | 155 | 179 | 659 | 2 | 0 | 0 | 1 |
| 27 | 249 | 157 | 181 | 829 | 0 | 0 | 0 | 1 |
| 26 | 258 | 166 | 190 | 553 | 0 | 0 | 2 | 1 |
| 25 | 262 | 170 | 194 | 834 | 2 | 0 | 0 | 1 |
| 24 | 263 | 171 | 195 | 560 | 0 | 0 | 0 | 1 |
| 23 | 265 | 173 | 197 | 602 | 13 | 0 | 0 | 1 |
| 22 | 268 | 176 | 200 | 794 | 0 | 0 | 2 | 1 |
| 21 | 272 | 180 | 204 | 580 | 0 | 0 | 0 | 1 |
| 20 | 273 | 181 | 205 | 425 | 0 | 0 | 84 | 1 |
| 19 | 274 | 182 | 206 | 0 | 0 | 0 | 634 | NaN |
The maximum aligned sequence corresponds to the maximum sequence covered by at least one correct sequencing.
Length of max aligned sequence : 147 bp
Coordinates of max aligned sequence :
chr16:68771007-68771153
Maximum aligned sequence (on bisulfite converted sense sequence from the plus strand sequence) :
## TTTTAGTAATTTTAGGTTAGAGGGTTATCGCGTTTATGCGAGGTCGGGTGGGCGGGTCGTTAGTTTCGTTTTGGGGAGGGGTTCGCGTTGTTGATTGGTTGTGGTCGGTAGGTGAATTTTTAGTTAATTAGCGGTACGGGGGGCGGT
| CG number | Chromosome | Start | End | Methylation Percentage Forward | Methylation Percentage Reverse | Methylation Percentage Average | Methylation Percentage SD |
|---|---|---|---|---|---|---|---|
| 17 | chr16 | 68771150 | 68771151 | NA | 100.00 | 100.00 | NA |
| 16 | chr16 | 68771143 | 68771144 | NA | 100.00 | 100.00 | NA |
| 15 | chr16 | 68771138 | 68771139 | NA | 100.00 | 100.00 | NA |
| 14 | chr16 | 68771112 | 68771113 | NA | 99.73 | 99.73 | NA |
| 13 | chr16 | 68771092 | 68771093 | NA | 100.00 | 100.00 | NA |
| 12 | chr16 | 68771090 | 68771091 | NA | 98.55 | 98.55 | NA |
| 11 | chr16 | 68771073 | 68771074 | NA | 77.83 | 77.83 | NA |
| 10 | chr16 | 68771064 | 68771065 | NA | 95.84 | 95.84 | NA |
| 9 | chr16 | 68771059 | 68771060 | NA | 100.00 | 100.00 | NA |
| 8 | chr16 | 68771051 | 68771052 | NA | 84.08 | 84.08 | NA |
| 7 | chr16 | 68771045 | 68771046 | NA | 100.00 | 100.00 | NA |
| 6 | chr16 | 68771037 | 68771038 | NA | 100.00 | 100.00 | NA |
| 5 | chr16 | 68771035 | 68771036 | NA | 83.50 | 83.50 | NA |
Warning: No sequencing data from foward direction sequencing.
| CG number | Chromosome | Start | End | G position in reference DNA | G position in sequencing result (trimmed) | G position in sequencing result (raw) | A peak | T peak | G peak | C peak | Methylation Percentage |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 17 | chr16 | 68771150 | 68771151 | 131 | 39 | 63 | 0 | 0 | 594 | 13 | 100.00 |
| 16 | chr16 | 68771143 | 68771144 | 138 | 46 | 70 | 0 | 0 | 687 | 0 | 100.00 |
| 15 | chr16 | 68771138 | 68771139 | 143 | 51 | 75 | 0 | 0 | 751 | 31 | 100.00 |
| 14 | chr16 | 68771112 | 68771113 | 169 | 77 | 101 | 2 | 0 | 743 | 2 | 99.73 |
| 13 | chr16 | 68771092 | 68771093 | 189 | 97 | 121 | 0 | 0 | 604 | 7 | 100.00 |
| 12 | chr16 | 68771090 | 68771091 | 191 | 99 | 123 | 7 | 0 | 477 | 0 | 98.55 |
| 11 | chr16 | 68771073 | 68771074 | 208 | 116 | 140 | 137 | 0 | 481 | 2 | 77.83 |
| 10 | chr16 | 68771064 | 68771065 | 217 | 125 | 149 | 29 | 0 | 668 | 2 | 95.84 |
| 9 | chr16 | 68771059 | 68771060 | 222 | 130 | 154 | 0 | 0 | 670 | 31 | 100.00 |
| 8 | chr16 | 68771051 | 68771052 | 230 | 138 | 162 | 121 | 0 | 639 | 0 | 84.08 |
| 7 | chr16 | 68771045 | 68771046 | 236 | 144 | 168 | 0 | 0 | 450 | 38 | 100.00 |
| 6 | chr16 | 68771037 | 68771038 | 244 | 152 | 176 | 0 | 0 | 530 | 32 | 100.00 |
| 5 | chr16 | 68771035 | 68771036 | 246 | 154 | 178 | 100 | 0 | 506 | 0 | 83.50 |
CDH1_High methylated DNA_2_aligned_DNAref_R.fasta
CDH1_High methylated DNA_2_aligned_max_seq.fasta
CDH1_High methylated DNA_2_chromatogram_1.pdf
CDH1_High methylated DNA_2_bisconv_table.csv
CDH1_High methylated DNA_2_bisconv_table.png
CDH1_High methylated DNA_2_bisconv_table.xlsx
CDH1_High methylated DNA_2_meth_table.csv
CDH1_High methylated DNA_2_meth_table.png
CDH1_High methylated DNA_2_meth_table.xlsx
CDH1_High methylated DNA_2_QC.png
CDH1_High methylated DNA_2_QC_table.csv
CDH1_High methylated DNA_2_QC_table.xlsx
CDH1_High methylated DNA_2_seqtable_1.csv
CDH1_High methylated DNA_2_seqtable_1.xlsx
CDH1_High methylated DNA_2_seqtable_2.csv
CDH1_High methylated DNA_2_seqtable_2.xlsx
CDH1_High methylated DNA_2_seqtable_trimmed_2.csv
CDH1_High methylated DNA_2_seqtable_trimmed_2.xlsx
CDH1_High methylated DNA_2_trimmedseq.png
| CG_nb | chromosome | start | end | meth_F | meth_R | meth_avg | meth_sd | collection | group | rep | clone | alg_coord_start | alg_coord_end |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 17 | chr16 | 68771150 | 68771151 | NA | 100.00 | 100.00 | NA | High methylated DNA | 2 | NA | 68771007 | 68771153 | |
| 16 | chr16 | 68771143 | 68771144 | NA | 100.00 | 100.00 | NA | High methylated DNA | 2 | NA | 68771007 | 68771153 | |
| 15 | chr16 | 68771138 | 68771139 | NA | 100.00 | 100.00 | NA | High methylated DNA | 2 | NA | 68771007 | 68771153 | |
| 14 | chr16 | 68771112 | 68771113 | NA | 99.73 | 99.73 | NA | High methylated DNA | 2 | NA | 68771007 | 68771153 | |
| 13 | chr16 | 68771092 | 68771093 | NA | 100.00 | 100.00 | NA | High methylated DNA | 2 | NA | 68771007 | 68771153 | |
| 12 | chr16 | 68771090 | 68771091 | NA | 98.55 | 98.55 | NA | High methylated DNA | 2 | NA | 68771007 | 68771153 | |
| 11 | chr16 | 68771073 | 68771074 | NA | 77.83 | 77.83 | NA | High methylated DNA | 2 | NA | 68771007 | 68771153 | |
| 10 | chr16 | 68771064 | 68771065 | NA | 95.84 | 95.84 | NA | High methylated DNA | 2 | NA | 68771007 | 68771153 | |
| 9 | chr16 | 68771059 | 68771060 | NA | 100.00 | 100.00 | NA | High methylated DNA | 2 | NA | 68771007 | 68771153 | |
| 8 | chr16 | 68771051 | 68771052 | NA | 84.08 | 84.08 | NA | High methylated DNA | 2 | NA | 68771007 | 68771153 | |
| 7 | chr16 | 68771045 | 68771046 | NA | 100.00 | 100.00 | NA | High methylated DNA | 2 | NA | 68771007 | 68771153 | |
| 6 | chr16 | 68771037 | 68771038 | NA | 100.00 | 100.00 | NA | High methylated DNA | 2 | NA | 68771007 | 68771153 | |
| 5 | chr16 | 68771035 | 68771036 | NA | 83.50 | 83.50 | NA | High methylated DNA | 2 | NA | 68771007 | 68771153 |